Normal Human Chromosome 1 Carries Suppressor Activity for Various Phenotypes of a Kirsten Murine Sarcoma Virus‐transformed NIH/3T3 Cell Line

In order to identify chromosomes that carry putative tumor‐suppressor genes for the various phenotypes of Kirsten sarcoma virus‐transformed NIH/3T3 (DT) cells, we performed microcell‐mediated chromosome transfer into DT cells. We first isolated mouse A9 clones, containing a single human chromosome 1, 11 or 12 tagged with pSV2‐neo plasmid DNA. Then, chromosome 1, 11 or 12 was transferred from the A9 clones into DT cells by microcell fusion. The growth rate, colony‐forming ability in soft agar and tumorigenicity of the DT cells were controlled by chromosome 1, but not by chromosome 11 or 12, indicating that normal human chromosome 1 carries a putative tumor‐suppressor gene(s) that affects various transformed phenotypes of DT cells.