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Detection of Proteins that Recognize Platinum‐modified DNA Using Gel Mobility Shift Assay

Using a gel mobility shift assay, we have identified proteins, in the nuclear extracts of a human lung cancer cell line, that recognize cis‐diamminedichloroplatinum(II) (cis‐DDP, CDDP)‐modified DNA. A 158‐base‐pair double‐stranded DNA fragment, derived from pBR322 plasmid DNA, was modified by either...

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Detalles Bibliográficos
Autores principales:	Fujiwara, Yasuhiro, Kasahara, Kazuo, Sugimoto, Yoshikazu, Nishio, Kazuto, Ohmori, Tohru, Saijo, Nagahiro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Blackwell Publishing Ltd 1990
Materias:	Rapid Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918002/ https://www.ncbi.nlm.nih.gov/pubmed/2125989 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02680.x

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