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Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5)

Acyclic retinoid (polyprenoic acid) has a slightly different structure from retinoic acid. However, acyclic retinoid acts similarly to retinoic acid, because both bind to cellular retinoic acid‐binding protein and cellular retinoid‐binding protein, F‐type, with the same strong binding affinity. We s...

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Detalles Bibliográficos
Autores principales: Fukutomi, Yasushi, Omori, Masahide, Muto, Yasutoshi, Ninomiya, Mitsuo, Okuno, Masataka, Moriwaki, Hisataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918005/
https://www.ncbi.nlm.nih.gov/pubmed/1703522
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02691.x
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author Fukutomi, Yasushi
Omori, Masahide
Muto, Yasutoshi
Ninomiya, Mitsuo
Okuno, Masataka
Moriwaki, Hisataka
author_facet Fukutomi, Yasushi
Omori, Masahide
Muto, Yasutoshi
Ninomiya, Mitsuo
Okuno, Masataka
Moriwaki, Hisataka
author_sort Fukutomi, Yasushi
collection PubMed
description Acyclic retinoid (polyprenoic acid) has a slightly different structure from retinoic acid. However, acyclic retinoid acts similarly to retinoic acid, because both bind to cellular retinoic acid‐binding protein and cellular retinoid‐binding protein, F‐type, with the same strong binding affinity. We studied the effects of acyclic retinoid, the 7‐hydroxy derivative of acyclic retinoid (7OH‐acyclic retinoid) and retinoic acid on a human hepatoma‐derived cell line PLC/PRF/5 (Alexander cells). Acyclic retinoid inhibited cell growth with an ID(50) value of 14 μM, and reduced cell viability with an LD(50) value of 86 μM. The ratios of LD(50) value to ID(50) value were 6.1 for acyclic retinoid, 2.4 for 7OH‐acyclic retinoid and 1.4 for all‐trans‐retinoic acid. Taking this ratio as a parameter of relative cytotoxicity, we concluded that acyclic retinoid is the least toxic compound. Growth inhibition of cells by acyclic retinoid was associated with the incorporation of (3)H‐thymidine in the logarithmic phase. Acyclic retinoid reduced secretion of α‐fetoprotein (AFP) and reciprocally increased secretion of albumin in the culture media, suggesting that acyclic retinoid influences gene expression of these proteins. Thus, acyclic retinoid, one of the less toxic retinoids, inhibits cell growth of human cancer cell line PLC/PRF/5 and appears to alter gene expression of AFP and albumin toward a “normal’direction.
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spelling pubmed-59180052018-05-11 Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5) Fukutomi, Yasushi Omori, Masahide Muto, Yasutoshi Ninomiya, Mitsuo Okuno, Masataka Moriwaki, Hisataka Jpn J Cancer Res Article Acyclic retinoid (polyprenoic acid) has a slightly different structure from retinoic acid. However, acyclic retinoid acts similarly to retinoic acid, because both bind to cellular retinoic acid‐binding protein and cellular retinoid‐binding protein, F‐type, with the same strong binding affinity. We studied the effects of acyclic retinoid, the 7‐hydroxy derivative of acyclic retinoid (7OH‐acyclic retinoid) and retinoic acid on a human hepatoma‐derived cell line PLC/PRF/5 (Alexander cells). Acyclic retinoid inhibited cell growth with an ID(50) value of 14 μM, and reduced cell viability with an LD(50) value of 86 μM. The ratios of LD(50) value to ID(50) value were 6.1 for acyclic retinoid, 2.4 for 7OH‐acyclic retinoid and 1.4 for all‐trans‐retinoic acid. Taking this ratio as a parameter of relative cytotoxicity, we concluded that acyclic retinoid is the least toxic compound. Growth inhibition of cells by acyclic retinoid was associated with the incorporation of (3)H‐thymidine in the logarithmic phase. Acyclic retinoid reduced secretion of α‐fetoprotein (AFP) and reciprocally increased secretion of albumin in the culture media, suggesting that acyclic retinoid influences gene expression of these proteins. Thus, acyclic retinoid, one of the less toxic retinoids, inhibits cell growth of human cancer cell line PLC/PRF/5 and appears to alter gene expression of AFP and albumin toward a “normal’direction. Blackwell Publishing Ltd 1990-12 /pmc/articles/PMC5918005/ /pubmed/1703522 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02691.x Text en
spellingShingle Article
Fukutomi, Yasushi
Omori, Masahide
Muto, Yasutoshi
Ninomiya, Mitsuo
Okuno, Masataka
Moriwaki, Hisataka
Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5)
title Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5)
title_full Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5)
title_fullStr Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5)
title_full_unstemmed Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5)
title_short Inhibitory Effects of Acyclic Retinoid (Polyprenoic Acid) and Its Hydroxy Derivative on Cell Growth and on Secretion of α‐Fetoprotein in Human Hepatoma‐derived Cell Line (PLC/PRF/5)
title_sort inhibitory effects of acyclic retinoid (polyprenoic acid) and its hydroxy derivative on cell growth and on secretion of α‐fetoprotein in human hepatoma‐derived cell line (plc/prf/5)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918005/
https://www.ncbi.nlm.nih.gov/pubmed/1703522
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02691.x
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