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Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver

Administration of butylated hydroxyanisole (BHA) orally at either 0.5 g or 1 g/kg daily for 14 days to rats did not produce any DNA adducts in the forestomach as measured by the (32)P‐postlabeling method using (1) limiting concentrations of (32)P‐ATP; (2) nuclease P(1) enhancement; or (3) butanol ex...

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Autores principales: Routledge, Michael N., Orton, Terry C., Lord, Peter G., Garner, R. Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918025/
https://www.ncbi.nlm.nih.gov/pubmed/2112524
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02553.x
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author Routledge, Michael N.
Orton, Terry C.
Lord, Peter G.
Garner, R. Colin
author_facet Routledge, Michael N.
Orton, Terry C.
Lord, Peter G.
Garner, R. Colin
author_sort Routledge, Michael N.
collection PubMed
description Administration of butylated hydroxyanisole (BHA) orally at either 0.5 g or 1 g/kg daily for 14 days to rats did not produce any DNA adducts in the forestomach as measured by the (32)P‐postlabeling method using (1) limiting concentrations of (32)P‐ATP; (2) nuclease P(1) enhancement; or (3) butanol extraction. Experiments were conducted to establish the effects of BHA administration on aristolochic acid (AA) DNA adduct formation in the forestomach and liver, when BHA was administered prior to, together with or after AA administration. Adduct levels per 10(9) nucleotides in the liver after oral dosing daily for 5 days with 1 mg/kg AA and BHA (1 g/kg) or corn oil (5 ml/kg) for 7 days were as follows: (a) BHA and AA given simultaneously; 235±71, (b) AA+corn oil; 63±39, (c) AA followed by BHA; 57±13, (d) AA followed by corn oil; 91±38, (e) BHA followed by AA; 90±12, (f) corn oil followed by AA; 83±24. For the forestomach the values were: (a) 236±86, (b) 77±25, (c) 367±97, (d) 296±47, (e) 217±81, (f) 70±64. These data suggest that BHA could have an enhancing effect on AA‐induced lesions in the forestomach if dosed together with, or prior to, AA as adduct levels are significantly higher than in controls.
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spelling pubmed-59180252018-05-11 Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver Routledge, Michael N. Orton, Terry C. Lord, Peter G. Garner, R. Colin Jpn J Cancer Res Article Administration of butylated hydroxyanisole (BHA) orally at either 0.5 g or 1 g/kg daily for 14 days to rats did not produce any DNA adducts in the forestomach as measured by the (32)P‐postlabeling method using (1) limiting concentrations of (32)P‐ATP; (2) nuclease P(1) enhancement; or (3) butanol extraction. Experiments were conducted to establish the effects of BHA administration on aristolochic acid (AA) DNA adduct formation in the forestomach and liver, when BHA was administered prior to, together with or after AA administration. Adduct levels per 10(9) nucleotides in the liver after oral dosing daily for 5 days with 1 mg/kg AA and BHA (1 g/kg) or corn oil (5 ml/kg) for 7 days were as follows: (a) BHA and AA given simultaneously; 235±71, (b) AA+corn oil; 63±39, (c) AA followed by BHA; 57±13, (d) AA followed by corn oil; 91±38, (e) BHA followed by AA; 90±12, (f) corn oil followed by AA; 83±24. For the forestomach the values were: (a) 236±86, (b) 77±25, (c) 367±97, (d) 296±47, (e) 217±81, (f) 70±64. These data suggest that BHA could have an enhancing effect on AA‐induced lesions in the forestomach if dosed together with, or prior to, AA as adduct levels are significantly higher than in controls. Blackwell Publishing Ltd 1990-03 /pmc/articles/PMC5918025/ /pubmed/2112524 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02553.x Text en
spellingShingle Article
Routledge, Michael N.
Orton, Terry C.
Lord, Peter G.
Garner, R. Colin
Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver
title Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver
title_full Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver
title_fullStr Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver
title_full_unstemmed Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver
title_short Effect of Butylated Hydroxyanisole on the Level of DNA Adduction by Aristolochic Acid in the Rat Forestomach and Liver
title_sort effect of butylated hydroxyanisole on the level of dna adduction by aristolochic acid in the rat forestomach and liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918025/
https://www.ncbi.nlm.nih.gov/pubmed/2112524
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02553.x
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