A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells

A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleoti...

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Detalles Bibliográficos
Autores principales: Harada, Fumio, Hirose, Yutaka, Tanaka, Motohiro, Sasaki, Takuma, Kamiya, Hiroyuki, Miura, Kazunobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1990
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918037/
https://www.ncbi.nlm.nih.gov/pubmed/2112525
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02555.x
Descripción
Sumario:A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.

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