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A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleoti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1990
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918037/ https://www.ncbi.nlm.nih.gov/pubmed/2112525 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02555.x |
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author | Harada, Fumio Hirose, Yutaka Tanaka, Motohiro Sasaki, Takuma Kamiya, Hiroyuki Miura, Kazunobu |
author_facet | Harada, Fumio Hirose, Yutaka Tanaka, Motohiro Sasaki, Takuma Kamiya, Hiroyuki Miura, Kazunobu |
author_sort | Harada, Fumio |
collection | PubMed |
description | A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues. |
format | Online Article Text |
id | pubmed-5918037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59180372018-05-11 A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells Harada, Fumio Hirose, Yutaka Tanaka, Motohiro Sasaki, Takuma Kamiya, Hiroyuki Miura, Kazunobu Jpn J Cancer Res Article A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues. Blackwell Publishing Ltd 1990-03 /pmc/articles/PMC5918037/ /pubmed/2112525 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02555.x Text en |
spellingShingle | Article Harada, Fumio Hirose, Yutaka Tanaka, Motohiro Sasaki, Takuma Kamiya, Hiroyuki Miura, Kazunobu A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells |
title | A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells |
title_full | A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells |
title_fullStr | A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells |
title_full_unstemmed | A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells |
title_short | A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells |
title_sort | simple, general method for detecting retroviral rnas expressed in cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918037/ https://www.ncbi.nlm.nih.gov/pubmed/2112525 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02555.x |
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