A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells

A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleoti...

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Detalles Bibliográficos
Autores principales: Harada, Fumio, Hirose, Yutaka, Tanaka, Motohiro, Sasaki, Takuma, Kamiya, Hiroyuki, Miura, Kazunobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1990
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918037/
https://www.ncbi.nlm.nih.gov/pubmed/2112525
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02555.x
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author Harada, Fumio
Hirose, Yutaka
Tanaka, Motohiro
Sasaki, Takuma
Kamiya, Hiroyuki
Miura, Kazunobu
author_facet Harada, Fumio
Hirose, Yutaka
Tanaka, Motohiro
Sasaki, Takuma
Kamiya, Hiroyuki
Miura, Kazunobu
author_sort Harada, Fumio
collection PubMed
description A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.
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spelling pubmed-59180372018-05-11 A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells Harada, Fumio Hirose, Yutaka Tanaka, Motohiro Sasaki, Takuma Kamiya, Hiroyuki Miura, Kazunobu Jpn J Cancer Res Article A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A(+) RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3′‐terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV‐I and HTLV‐II in virus‐infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)‐derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues. Blackwell Publishing Ltd 1990-03 /pmc/articles/PMC5918037/ /pubmed/2112525 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02555.x Text en
spellingShingle Article
Harada, Fumio
Hirose, Yutaka
Tanaka, Motohiro
Sasaki, Takuma
Kamiya, Hiroyuki
Miura, Kazunobu
A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
title A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
title_full A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
title_fullStr A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
title_full_unstemmed A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
title_short A Simple, General Method for Detecting Retroviral RNAs Expressed in Cells
title_sort simple, general method for detecting retroviral rnas expressed in cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918037/
https://www.ncbi.nlm.nih.gov/pubmed/2112525
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02555.x
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