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Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells
Phosphorylation in normal and transformed NIH3T3 cells of the 80K protein, a specific substrate for protein kinase C, was compared by means of two‐dimensional gel analysis. We obtained evidence that NIH3T3 cells transformed by the c‐raf or H‐ras oncogene maintained a decreased level of phosphorylati...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1990
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918085/ https://www.ncbi.nlm.nih.gov/pubmed/2118892 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02648.x |
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author | Oh‐uchida, Mamoru Yano, Kazumi Kawamoto, Shoko Shimizu, Kenji |
author_facet | Oh‐uchida, Mamoru Yano, Kazumi Kawamoto, Shoko Shimizu, Kenji |
author_sort | Oh‐uchida, Mamoru |
collection | PubMed |
description | Phosphorylation in normal and transformed NIH3T3 cells of the 80K protein, a specific substrate for protein kinase C, was compared by means of two‐dimensional gel analysis. We obtained evidence that NIH3T3 cells transformed by the c‐raf or H‐ras oncogene maintained a decreased level of phosphorylation of the 80K protein, with or without phorbol ester (TPA)‐stimulation, at all concentrations of serum tested while normal NIH3T3 cells maintained an elevated level of phosphorylation of the 80K protein. Furthermore, NIH3T3 cells transformed by N‐ras, K‐ras, src, mos or polyoma middle T antigen exhibited a decreased level of phosphorylation of the 80K protein. These events were confirmed by an analysis of a hormone‐inducible H‐ras transformant. Thus, phosphorylation of the 80K protein is inversely correlated with cellular transformation. |
format | Online Article Text |
id | pubmed-5918085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59180852018-05-11 Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells Oh‐uchida, Mamoru Yano, Kazumi Kawamoto, Shoko Shimizu, Kenji Jpn J Cancer Res Article Phosphorylation in normal and transformed NIH3T3 cells of the 80K protein, a specific substrate for protein kinase C, was compared by means of two‐dimensional gel analysis. We obtained evidence that NIH3T3 cells transformed by the c‐raf or H‐ras oncogene maintained a decreased level of phosphorylation of the 80K protein, with or without phorbol ester (TPA)‐stimulation, at all concentrations of serum tested while normal NIH3T3 cells maintained an elevated level of phosphorylation of the 80K protein. Furthermore, NIH3T3 cells transformed by N‐ras, K‐ras, src, mos or polyoma middle T antigen exhibited a decreased level of phosphorylation of the 80K protein. These events were confirmed by an analysis of a hormone‐inducible H‐ras transformant. Thus, phosphorylation of the 80K protein is inversely correlated with cellular transformation. Blackwell Publishing Ltd 1990-08 /pmc/articles/PMC5918085/ /pubmed/2118892 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02648.x Text en |
spellingShingle | Article Oh‐uchida, Mamoru Yano, Kazumi Kawamoto, Shoko Shimizu, Kenji Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells |
title | Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells |
title_full | Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells |
title_fullStr | Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells |
title_full_unstemmed | Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells |
title_short | Transformation‐specific Decrease of Phosphorylation of 80K Protein, a Substrate of Protein Kinase C, in NIH3T3 Cells |
title_sort | transformation‐specific decrease of phosphorylation of 80k protein, a substrate of protein kinase c, in nih3t3 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918085/ https://www.ncbi.nlm.nih.gov/pubmed/2118892 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02648.x |
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