Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin

The mechanism of cell damage by ultrasound in combination with hematoporphyrin was studied. Mouse sarcoma 180 cell suspensions were exposed to ultrasound for up to 60 s in the presence and absence of hematoporphyrin, with and without active oxygen scavengers. The cell damage enhancement by hematopor...

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Autores principales: Umemura, Shin‐ichiro, Yumita, Nagahiko, Nishigaki, Ryuichiro, Umemura, Koshiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1990
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918111/
https://www.ncbi.nlm.nih.gov/pubmed/2172198
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02674.x
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author Umemura, Shin‐ichiro
Yumita, Nagahiko
Nishigaki, Ryuichiro
Umemura, Koshiro
author_facet Umemura, Shin‐ichiro
Yumita, Nagahiko
Nishigaki, Ryuichiro
Umemura, Koshiro
author_sort Umemura, Shin‐ichiro
collection PubMed
description The mechanism of cell damage by ultrasound in combination with hematoporphyrin was studied. Mouse sarcoma 180 cell suspensions were exposed to ultrasound for up to 60 s in the presence and absence of hematoporphyrin, with and without active oxygen scavengers. The cell damage enhancement by hematoporphyrin was suppressed by adding histidine but not by mannitol. The enhancement was doubled in rate by substitution of deuterium oxide medium for normal water. Sonoluminescence was produced in a saline solution under similar acoustic conditions and observed to have spectral components that can excite hematoporphyrin molecules. These results suggest that cell damage enhancement is probably mediated via singlet oxygen generated by ultrasonically activated hematoporphyrin.
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spelling pubmed-59181112018-05-11 Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin Umemura, Shin‐ichiro Yumita, Nagahiko Nishigaki, Ryuichiro Umemura, Koshiro Jpn J Cancer Res Article The mechanism of cell damage by ultrasound in combination with hematoporphyrin was studied. Mouse sarcoma 180 cell suspensions were exposed to ultrasound for up to 60 s in the presence and absence of hematoporphyrin, with and without active oxygen scavengers. The cell damage enhancement by hematoporphyrin was suppressed by adding histidine but not by mannitol. The enhancement was doubled in rate by substitution of deuterium oxide medium for normal water. Sonoluminescence was produced in a saline solution under similar acoustic conditions and observed to have spectral components that can excite hematoporphyrin molecules. These results suggest that cell damage enhancement is probably mediated via singlet oxygen generated by ultrasonically activated hematoporphyrin. Blackwell Publishing Ltd 1990-09 /pmc/articles/PMC5918111/ /pubmed/2172198 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02674.x Text en
spellingShingle Article
Umemura, Shin‐ichiro
Yumita, Nagahiko
Nishigaki, Ryuichiro
Umemura, Koshiro
Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin
title Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin
title_full Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin
title_fullStr Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin
title_full_unstemmed Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin
title_short Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin
title_sort mechanism of cell damage by ultrasound in combination with hematoporphyrin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918111/
https://www.ncbi.nlm.nih.gov/pubmed/2172198
http://dx.doi.org/10.1111/j.1349-7006.1990.tb02674.x
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