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In situ Localization of the Human Multidrug‐resistance Gene mRNA Using Thymine‐Thymine Dimerized Single‐stranded cDNA

In order to detect the mRNA transcribed from the multidrug‐resistance gene (MDR1), thymine‐thymine (T‐T) dimerized single‐stranded DNA probes have been utilized for hybridization with mRNA either on nitrocellulose filters or in cells and tissues. S1 nuclease digestion rather than sonication was used...

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Detalles Bibliográficos
Autores principales:	Sugawara, Isamu, Koji, Takehiko, Ueda, Kazumitsu, Pastan, Ira, Gottesman, Michael M., Nakane, Paul K., Mori, Shigeo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Blackwell Publishing Ltd 1990
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918119/ https://www.ncbi.nlm.nih.gov/pubmed/1977730 http://dx.doi.org/10.1111/j.1349-7006.1990.tb02672.x

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