Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein

We examined 500 human sera for the presence of antibody against the human papillomavirus type 16 E7 protein by enzyme‐linked immunosorbent assay with bacterially expressed fusion protein lac‐E7, and by radioimmunoprecipitation and immunofluorescence assays with the E7 protein expressed in monkey COS...

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Autores principales: Hashido, Madoka, Kanda, Tadahito, Zanma, Soichi, Watanabe, Sumie, Komiyama, Naoki, Yoshikawa, Hiroyuki, Yamaguchi, Nobuo, Kawana, Takashi, Kunito, Kunito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1991
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918352/
https://www.ncbi.nlm.nih.gov/pubmed/1663920
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01813.x
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author Hashido, Madoka
Kanda, Tadahito
Zanma, Soichi
Watanabe, Sumie
Komiyama, Naoki
Yoshikawa, Hiroyuki
Yamaguchi, Nobuo
Kawana, Takashi
Kunito, Kunito
author_facet Hashido, Madoka
Kanda, Tadahito
Zanma, Soichi
Watanabe, Sumie
Komiyama, Naoki
Yoshikawa, Hiroyuki
Yamaguchi, Nobuo
Kawana, Takashi
Kunito, Kunito
author_sort Hashido, Madoka
collection PubMed
description We examined 500 human sera for the presence of antibody against the human papillomavirus type 16 E7 protein by enzyme‐linked immunosorbent assay with bacterially expressed fusion protein lac‐E7, and by radioimmunoprecipitation and immunofluorescence assays with the E7 protein expressed in monkey COS‐1 cells. The anti‐E7 antibody was detectable in 6 out of 54 cases of cervical carcinoma, but in none of the others, including patients with other gynecologic cancers, those with sexually transmitted diseases, and healthy adults. The data indicate that expression of the E7 protein is closely related to cervical carcinoma.
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spelling pubmed-59183522018-05-11 Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein Hashido, Madoka Kanda, Tadahito Zanma, Soichi Watanabe, Sumie Komiyama, Naoki Yoshikawa, Hiroyuki Yamaguchi, Nobuo Kawana, Takashi Kunito, Kunito Jpn J Cancer Res Article We examined 500 human sera for the presence of antibody against the human papillomavirus type 16 E7 protein by enzyme‐linked immunosorbent assay with bacterially expressed fusion protein lac‐E7, and by radioimmunoprecipitation and immunofluorescence assays with the E7 protein expressed in monkey COS‐1 cells. The anti‐E7 antibody was detectable in 6 out of 54 cases of cervical carcinoma, but in none of the others, including patients with other gynecologic cancers, those with sexually transmitted diseases, and healthy adults. The data indicate that expression of the E7 protein is closely related to cervical carcinoma. Blackwell Publishing Ltd 1991-12 /pmc/articles/PMC5918352/ /pubmed/1663920 http://dx.doi.org/10.1111/j.1349-7006.1991.tb01813.x Text en
spellingShingle Article
Hashido, Madoka
Kanda, Tadahito
Zanma, Soichi
Watanabe, Sumie
Komiyama, Naoki
Yoshikawa, Hiroyuki
Yamaguchi, Nobuo
Kawana, Takashi
Kunito, Kunito
Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein
title Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein
title_full Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein
title_fullStr Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein
title_full_unstemmed Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein
title_short Detection of Human Antibody against the Human Papillomavirus Type 16 E7 Protein
title_sort detection of human antibody against the human papillomavirus type 16 e7 protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918352/
https://www.ncbi.nlm.nih.gov/pubmed/1663920
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01813.x
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