Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen

The antigen detected by monoclonal antibodies reacting with human osteosarcoma‐associated antigen was shown to he a phosphatidyl‐inositol (Pl)‐glycan‐anchored protein, which can be released from the cell surface by PI‐specific phospholipase C‐treatment. The antigen detected by 2D3 and 2H10 antibodie...

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Autores principales: Nagoya, Satoshi, Uede, Toshimitsu, Wada, Takuro, Ishii, Seiichi, Yamawaki, Shinya, Kikuchi, Kokichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1991
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918541/
https://www.ncbi.nlm.nih.gov/pubmed/1715340
http://dx.doi.org/10.1111/j.1349-7006.1991.tb02714.x
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author Nagoya, Satoshi
Uede, Toshimitsu
Wada, Takuro
Ishii, Seiichi
Yamawaki, Shinya
Kikuchi, Kokichi
author_facet Nagoya, Satoshi
Uede, Toshimitsu
Wada, Takuro
Ishii, Seiichi
Yamawaki, Shinya
Kikuchi, Kokichi
author_sort Nagoya, Satoshi
collection PubMed
description The antigen detected by monoclonal antibodies reacting with human osteosarcoma‐associated antigen was shown to he a phosphatidyl‐inositol (Pl)‐glycan‐anchored protein, which can be released from the cell surface by PI‐specific phospholipase C‐treatment. The antigen detected by 2D3 and 2H10 antibodies exhibited alkaline phosphatase activity. Both antibodies strongly reacted with bone‐type alkaline phosphatase. However, importantly, immunohistochemical analysis demonstrated that 2D3 and 2H10 did not react with alkaline phosphatase present in kidney or liver. In addition, neither placental nor intestinal alkaline phosphatase was recognized by 2D3 and 2H10 antibodies. These results indicated that two monoclonal antibodies, 2D3 and 2H10, are highly specific for bone‐type alkaline phosphatase and can distinguish bone alkaline phosphatase from liver alkaline phosphatase in spite of the fact that liver and bone alkaline phosphatase are encoded by the same gene.
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spelling pubmed-59185412018-05-11 Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen Nagoya, Satoshi Uede, Toshimitsu Wada, Takuro Ishii, Seiichi Yamawaki, Shinya Kikuchi, Kokichi Jpn J Cancer Res Article The antigen detected by monoclonal antibodies reacting with human osteosarcoma‐associated antigen was shown to he a phosphatidyl‐inositol (Pl)‐glycan‐anchored protein, which can be released from the cell surface by PI‐specific phospholipase C‐treatment. The antigen detected by 2D3 and 2H10 antibodies exhibited alkaline phosphatase activity. Both antibodies strongly reacted with bone‐type alkaline phosphatase. However, importantly, immunohistochemical analysis demonstrated that 2D3 and 2H10 did not react with alkaline phosphatase present in kidney or liver. In addition, neither placental nor intestinal alkaline phosphatase was recognized by 2D3 and 2H10 antibodies. These results indicated that two monoclonal antibodies, 2D3 and 2H10, are highly specific for bone‐type alkaline phosphatase and can distinguish bone alkaline phosphatase from liver alkaline phosphatase in spite of the fact that liver and bone alkaline phosphatase are encoded by the same gene. Blackwell Publishing Ltd 1991-07 /pmc/articles/PMC5918541/ /pubmed/1715340 http://dx.doi.org/10.1111/j.1349-7006.1991.tb02714.x Text en
spellingShingle Article
Nagoya, Satoshi
Uede, Toshimitsu
Wada, Takuro
Ishii, Seiichi
Yamawaki, Shinya
Kikuchi, Kokichi
Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen
title Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen
title_full Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen
title_fullStr Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen
title_full_unstemmed Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen
title_short Detection of Bone‐type Alkaline Phosphatase by Monoclonal Antibodies Reacting with Human Osteosarcoma‐associated Antigen
title_sort detection of bone‐type alkaline phosphatase by monoclonal antibodies reacting with human osteosarcoma‐associated antigen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918541/
https://www.ncbi.nlm.nih.gov/pubmed/1715340
http://dx.doi.org/10.1111/j.1349-7006.1991.tb02714.x
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