Cargando…

Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus

BACKGROUND: Infections of sheep with gastrointestinal parasites, especially Haemonchus contortus, have caused serious losses in livestock production, particularly after the emergence of resistance to conventional anthelmintics. The search for new anthelmintic agents, especially those of botanical or...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferreira, Luis Eduardo, Benincasa, Bruno Iglesias, Fachin, Ana Lúcia, Contini, Silvia Helena Taleb, França, Suzelei Castro, Chagas, Ana Carolina Souza, Beleboni, Rene Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918559/
https://www.ncbi.nlm.nih.gov/pubmed/29695271
http://dx.doi.org/10.1186/s13071-018-2849-x
_version_ 1783317443592060928
author Ferreira, Luis Eduardo
Benincasa, Bruno Iglesias
Fachin, Ana Lúcia
Contini, Silvia Helena Taleb
França, Suzelei Castro
Chagas, Ana Carolina Souza
Beleboni, Rene Oliveira
author_facet Ferreira, Luis Eduardo
Benincasa, Bruno Iglesias
Fachin, Ana Lúcia
Contini, Silvia Helena Taleb
França, Suzelei Castro
Chagas, Ana Carolina Souza
Beleboni, Rene Oliveira
author_sort Ferreira, Luis Eduardo
collection PubMed
description BACKGROUND: Infections of sheep with gastrointestinal parasites, especially Haemonchus contortus, have caused serious losses in livestock production, particularly after the emergence of resistance to conventional anthelmintics. The search for new anthelmintic agents, especially those of botanical origin, has grown substantially due to the perspective of less contamination of meat and milk, as well as other advantages related to their cost and accessibility in less developed countries. The aim of this study was to evaluate the in vitro anthelmintic activity of essential oils of the plant species Citrus aurantifolia, Anthemis nobile and Lavandula officinalis against the main developmental stages of the parasite H. contortus. RESULTS: Plant species were selected based on substantial ethnopharmacological information. Analysis of the composition of each oil by gas chromatography coupled to mass spectrometry (GC-MS) demonstrated the presence of limonene (56.37%), isobutyl angelate (29.26%) and linalool acetate (35.97%) as the major constituents in C. aurantifolia, A. nobile and L. officinalis, respectively. Different concentrations of each oil were tested in vitro for their capacity to inhibit egg hatching (EHT), larval development (LDT) and adult worm motility (AWMT) using a multidrug-resistant strain of H. contortus (Embrapa 2010). The IC(50) values obtained for the oils of C. aurantifolia, A. nobile and L. officinalis were 0.694, 0.842 and 0.316 mg/ml in the EHT and 0.044, 0.117 and 0.280 mg/ml in the LDT, respectively. The three oils were able to inhibit adult worm motility completely within the first 8–12 h of observation in the AWMT. CONCLUSIONS: The present results demonstrate significant anthelmintic activity of the three oils against the different developmental stages of H. contortus. Furthermore, this study is of ethnopharmacological importance by validating the anthelmintic activity of the oils studied. Although new experiments are necessary, these data contribute to the development of pharmaceutical-veterinary products for sheep farming by opening up new therapeutic possibilities against gastrointestinal infections caused by H. contortus.
format Online
Article
Text
id pubmed-5918559
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-59185592018-04-30 Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus Ferreira, Luis Eduardo Benincasa, Bruno Iglesias Fachin, Ana Lúcia Contini, Silvia Helena Taleb França, Suzelei Castro Chagas, Ana Carolina Souza Beleboni, Rene Oliveira Parasit Vectors Research BACKGROUND: Infections of sheep with gastrointestinal parasites, especially Haemonchus contortus, have caused serious losses in livestock production, particularly after the emergence of resistance to conventional anthelmintics. The search for new anthelmintic agents, especially those of botanical origin, has grown substantially due to the perspective of less contamination of meat and milk, as well as other advantages related to their cost and accessibility in less developed countries. The aim of this study was to evaluate the in vitro anthelmintic activity of essential oils of the plant species Citrus aurantifolia, Anthemis nobile and Lavandula officinalis against the main developmental stages of the parasite H. contortus. RESULTS: Plant species were selected based on substantial ethnopharmacological information. Analysis of the composition of each oil by gas chromatography coupled to mass spectrometry (GC-MS) demonstrated the presence of limonene (56.37%), isobutyl angelate (29.26%) and linalool acetate (35.97%) as the major constituents in C. aurantifolia, A. nobile and L. officinalis, respectively. Different concentrations of each oil were tested in vitro for their capacity to inhibit egg hatching (EHT), larval development (LDT) and adult worm motility (AWMT) using a multidrug-resistant strain of H. contortus (Embrapa 2010). The IC(50) values obtained for the oils of C. aurantifolia, A. nobile and L. officinalis were 0.694, 0.842 and 0.316 mg/ml in the EHT and 0.044, 0.117 and 0.280 mg/ml in the LDT, respectively. The three oils were able to inhibit adult worm motility completely within the first 8–12 h of observation in the AWMT. CONCLUSIONS: The present results demonstrate significant anthelmintic activity of the three oils against the different developmental stages of H. contortus. Furthermore, this study is of ethnopharmacological importance by validating the anthelmintic activity of the oils studied. Although new experiments are necessary, these data contribute to the development of pharmaceutical-veterinary products for sheep farming by opening up new therapeutic possibilities against gastrointestinal infections caused by H. contortus. BioMed Central 2018-04-25 /pmc/articles/PMC5918559/ /pubmed/29695271 http://dx.doi.org/10.1186/s13071-018-2849-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ferreira, Luis Eduardo
Benincasa, Bruno Iglesias
Fachin, Ana Lúcia
Contini, Silvia Helena Taleb
França, Suzelei Castro
Chagas, Ana Carolina Souza
Beleboni, Rene Oliveira
Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus
title Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus
title_full Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus
title_fullStr Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus
title_full_unstemmed Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus
title_short Essential oils of Citrus aurantifolia, Anthemis nobile and Lavandula officinalis: in vitro anthelmintic activities against Haemonchus contortus
title_sort essential oils of citrus aurantifolia, anthemis nobile and lavandula officinalis: in vitro anthelmintic activities against haemonchus contortus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918559/
https://www.ncbi.nlm.nih.gov/pubmed/29695271
http://dx.doi.org/10.1186/s13071-018-2849-x
work_keys_str_mv AT ferreiraluiseduardo essentialoilsofcitrusaurantifoliaanthemisnobileandlavandulaofficinalisinvitroanthelminticactivitiesagainsthaemonchuscontortus
AT benincasabrunoiglesias essentialoilsofcitrusaurantifoliaanthemisnobileandlavandulaofficinalisinvitroanthelminticactivitiesagainsthaemonchuscontortus
AT fachinanalucia essentialoilsofcitrusaurantifoliaanthemisnobileandlavandulaofficinalisinvitroanthelminticactivitiesagainsthaemonchuscontortus
AT continisilviahelenataleb essentialoilsofcitrusaurantifoliaanthemisnobileandlavandulaofficinalisinvitroanthelminticactivitiesagainsthaemonchuscontortus
AT francasuzeleicastro essentialoilsofcitrusaurantifoliaanthemisnobileandlavandulaofficinalisinvitroanthelminticactivitiesagainsthaemonchuscontortus
AT chagasanacarolinasouza essentialoilsofcitrusaurantifoliaanthemisnobileandlavandulaofficinalisinvitroanthelminticactivitiesagainsthaemonchuscontortus
AT belebonireneoliveira essentialoilsofcitrusaurantifoliaanthemisnobileandlavandulaofficinalisinvitroanthelminticactivitiesagainsthaemonchuscontortus