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Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model

BACKGROUND: In recent years, cancer rates have been rising among reproductive-age women. Thus, chemotherapy exposure has become an important cause of premature ovarian failure (POF). There has been growing interest regarding the preservation and restoration of ovarian function before and after oncol...

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Autores principales: Melekoglu, Rauf, Ciftci, Osman, Eraslan, Sevil, Cetin, Asli, Basak, Nese
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918567/
https://www.ncbi.nlm.nih.gov/pubmed/29699594
http://dx.doi.org/10.1186/s13048-018-0409-9
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author Melekoglu, Rauf
Ciftci, Osman
Eraslan, Sevil
Cetin, Asli
Basak, Nese
author_facet Melekoglu, Rauf
Ciftci, Osman
Eraslan, Sevil
Cetin, Asli
Basak, Nese
author_sort Melekoglu, Rauf
collection PubMed
description BACKGROUND: In recent years, cancer rates have been rising among reproductive-age women. Thus, chemotherapy exposure has become an important cause of premature ovarian failure (POF). There has been growing interest regarding the preservation and restoration of ovarian function before and after oncological treatment because of the reproductive risk of chemotherapeutics and improved long-term survival of cancer patients. In this study, we sought to analyze the effects of curcumin (CRC) and capsaicin (CPS) on cyclophosphamide-induced POF in a rat model. METHODS: POF in rats was induced by intraperitoneal injection of 200 mg/kg cyclophosphamide on day 1 and then 8 mg/kg/day for the following 14 days. After 14 days of cyclophosphamide administration, rats were randomly divided into three groups as follows (n = 10/group): POF, POF + CRC (100 mg/kg/day), and POF + CPS (0.5 mg/kg/day) to determine the effects of CRC and CPS on the cyclophosphamide-induced POF rat model. Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after the CRC and CPS treatments. RESULTS: Malonaldehyde levels were significantly reduced, and glutathione levels and superoxide dismutase activity were significantly increased, in ovarian tissues in the POF + CRC and POF + CPS groups compared with the POF group. In the POF group, we observed hemorrhage and prominent mononuclear cell infiltration beneath the germinative epithelium, vascular congestion in ovarian stroma, hemorrhage around the corpus luteum, and atresia in ovarian follicles. This histopathological damage was significantly improved by treatment with CRC and CPS. There was a significant reduction in serum follicle-stimulating hormone and luteinizing hormone levels in rats treated with CRC and CPS compared with the POF group. Moreover, the levels of estradiol and anti-mullerian hormone in rats treated with CRC and CPS were significantly increased compared with the control group. CONCLUSIONS: In conclusion, CRC and CPS treatment of rats with cyclophosphamide-induced POF had a beneficial effect on reducing ovarian damage by improving tissue oxidative stress marker levels, ovarian reserve marker levels, and histopathological parameters. The significant improvements in ovarian tissue histopathological damage and hormonal levels detected in this study indicate that treatment with CRC or CPS might be a conservative treatment approach for cyclophosphamide-induced POF.
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spelling pubmed-59185672018-04-30 Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model Melekoglu, Rauf Ciftci, Osman Eraslan, Sevil Cetin, Asli Basak, Nese J Ovarian Res Research BACKGROUND: In recent years, cancer rates have been rising among reproductive-age women. Thus, chemotherapy exposure has become an important cause of premature ovarian failure (POF). There has been growing interest regarding the preservation and restoration of ovarian function before and after oncological treatment because of the reproductive risk of chemotherapeutics and improved long-term survival of cancer patients. In this study, we sought to analyze the effects of curcumin (CRC) and capsaicin (CPS) on cyclophosphamide-induced POF in a rat model. METHODS: POF in rats was induced by intraperitoneal injection of 200 mg/kg cyclophosphamide on day 1 and then 8 mg/kg/day for the following 14 days. After 14 days of cyclophosphamide administration, rats were randomly divided into three groups as follows (n = 10/group): POF, POF + CRC (100 mg/kg/day), and POF + CPS (0.5 mg/kg/day) to determine the effects of CRC and CPS on the cyclophosphamide-induced POF rat model. Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after the CRC and CPS treatments. RESULTS: Malonaldehyde levels were significantly reduced, and glutathione levels and superoxide dismutase activity were significantly increased, in ovarian tissues in the POF + CRC and POF + CPS groups compared with the POF group. In the POF group, we observed hemorrhage and prominent mononuclear cell infiltration beneath the germinative epithelium, vascular congestion in ovarian stroma, hemorrhage around the corpus luteum, and atresia in ovarian follicles. This histopathological damage was significantly improved by treatment with CRC and CPS. There was a significant reduction in serum follicle-stimulating hormone and luteinizing hormone levels in rats treated with CRC and CPS compared with the POF group. Moreover, the levels of estradiol and anti-mullerian hormone in rats treated with CRC and CPS were significantly increased compared with the control group. CONCLUSIONS: In conclusion, CRC and CPS treatment of rats with cyclophosphamide-induced POF had a beneficial effect on reducing ovarian damage by improving tissue oxidative stress marker levels, ovarian reserve marker levels, and histopathological parameters. The significant improvements in ovarian tissue histopathological damage and hormonal levels detected in this study indicate that treatment with CRC or CPS might be a conservative treatment approach for cyclophosphamide-induced POF. BioMed Central 2018-04-26 /pmc/articles/PMC5918567/ /pubmed/29699594 http://dx.doi.org/10.1186/s13048-018-0409-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Melekoglu, Rauf
Ciftci, Osman
Eraslan, Sevil
Cetin, Asli
Basak, Nese
Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
title Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
title_full Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
title_fullStr Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
title_full_unstemmed Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
title_short Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
title_sort beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918567/
https://www.ncbi.nlm.nih.gov/pubmed/29699594
http://dx.doi.org/10.1186/s13048-018-0409-9
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