Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media

A new cell line, designated Ist‐1, was established in serum‐free medium from a 63‐year‐old gastric cancer patient with an extremely high serum level of α‐fetoprotein (AFP). ISt‐1 exhibited a typical morphology of epithelial cells in culture. The population doubling time was 31 hours. Production of A...

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Autores principales: Terashima, Masanori, Ikeda, Kenichiro, Maesawa, Chihaya, Kawamura, Hidenobu, Niitsu, Yorikazu, Satoh, Masao, Saito, Kazuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1991
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918585/
https://www.ncbi.nlm.nih.gov/pubmed/1716623
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01915.x
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author Terashima, Masanori
Ikeda, Kenichiro
Maesawa, Chihaya
Kawamura, Hidenobu
Niitsu, Yorikazu
Satoh, Masao
Saito, Kazuyoshi
author_facet Terashima, Masanori
Ikeda, Kenichiro
Maesawa, Chihaya
Kawamura, Hidenobu
Niitsu, Yorikazu
Satoh, Masao
Saito, Kazuyoshi
author_sort Terashima, Masanori
collection PubMed
description A new cell line, designated Ist‐1, was established in serum‐free medium from a 63‐year‐old gastric cancer patient with an extremely high serum level of α‐fetoprotein (AFP). ISt‐1 exhibited a typical morphology of epithelial cells in culture. The population doubling time was 31 hours. Production of AFP was demonstrated by immmunohistochemical study and high levels of AFP were detected in the conditioned medium of ISt‐1 cells. Ist‐1 is considered to be an AFP‐producing gastric cancer cell line and will provide a useful tool for the study of production and secretion of AFP from cells.
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spelling pubmed-59185852018-05-11 Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media Terashima, Masanori Ikeda, Kenichiro Maesawa, Chihaya Kawamura, Hidenobu Niitsu, Yorikazu Satoh, Masao Saito, Kazuyoshi Jpn J Cancer Res Rapid Communication A new cell line, designated Ist‐1, was established in serum‐free medium from a 63‐year‐old gastric cancer patient with an extremely high serum level of α‐fetoprotein (AFP). ISt‐1 exhibited a typical morphology of epithelial cells in culture. The population doubling time was 31 hours. Production of AFP was demonstrated by immmunohistochemical study and high levels of AFP were detected in the conditioned medium of ISt‐1 cells. Ist‐1 is considered to be an AFP‐producing gastric cancer cell line and will provide a useful tool for the study of production and secretion of AFP from cells. Blackwell Publishing Ltd 1991-08 /pmc/articles/PMC5918585/ /pubmed/1716623 http://dx.doi.org/10.1111/j.1349-7006.1991.tb01915.x Text en
spellingShingle Rapid Communication
Terashima, Masanori
Ikeda, Kenichiro
Maesawa, Chihaya
Kawamura, Hidenobu
Niitsu, Yorikazu
Satoh, Masao
Saito, Kazuyoshi
Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media
title Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media
title_full Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media
title_fullStr Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media
title_full_unstemmed Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media
title_short Establishment of an α‐Fetoprotein‐producing Gastric Cancer Cell Line in Serum‐free Media
title_sort establishment of an α‐fetoprotein‐producing gastric cancer cell line in serum‐free media
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918585/
https://www.ncbi.nlm.nih.gov/pubmed/1716623
http://dx.doi.org/10.1111/j.1349-7006.1991.tb01915.x
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