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Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine
Five pancreatic carcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki‐ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki‐ras gene was shown to be activated in four of the five carcinomas, and the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1991
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918598/ https://www.ncbi.nlm.nih.gov/pubmed/1657847 http://dx.doi.org/10.1111/j.1349-7006.1991.tb01928.x |
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author | Ushijima, Toshikazu Tsutsumi, Masahiro Sakai, Ryuichi Ishizaka, Yukihito Takaku, Fumimaro Konishi, Yoichi Takahashi, Michihito Sugimura, Takashi Nagao, Minako |
author_facet | Ushijima, Toshikazu Tsutsumi, Masahiro Sakai, Ryuichi Ishizaka, Yukihito Takaku, Fumimaro Konishi, Yoichi Takahashi, Michihito Sugimura, Takashi Nagao, Minako |
author_sort | Ushijima, Toshikazu |
collection | PubMed |
description | Five pancreatic carcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki‐ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki‐ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G‐to‐A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N‐nitrosobis(2‐oxopropyl)amine. |
format | Online Article Text |
id | pubmed-5918598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1991 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59185982018-05-11 Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine Ushijima, Toshikazu Tsutsumi, Masahiro Sakai, Ryuichi Ishizaka, Yukihito Takaku, Fumimaro Konishi, Yoichi Takahashi, Michihito Sugimura, Takashi Nagao, Minako Jpn J Cancer Res Rapid Communication Five pancreatic carcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki‐ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki‐ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G‐to‐A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N‐nitrosobis(2‐oxopropyl)amine. Blackwell Publishing Ltd 1991-09 /pmc/articles/PMC5918598/ /pubmed/1657847 http://dx.doi.org/10.1111/j.1349-7006.1991.tb01928.x Text en |
spellingShingle | Rapid Communication Ushijima, Toshikazu Tsutsumi, Masahiro Sakai, Ryuichi Ishizaka, Yukihito Takaku, Fumimaro Konishi, Yoichi Takahashi, Michihito Sugimura, Takashi Nagao, Minako Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine |
title | Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine |
title_full | Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine |
title_fullStr | Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine |
title_full_unstemmed | Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine |
title_short | Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine |
title_sort | ki‐ras activation in pancreatic carcinomas of syrian hamsters induced by n‐nitrosobis(2‐hydroxypropyl)amine |
topic | Rapid Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918598/ https://www.ncbi.nlm.nih.gov/pubmed/1657847 http://dx.doi.org/10.1111/j.1349-7006.1991.tb01928.x |
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