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Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients

Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete...

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Autores principales: Yagyu, Toshio, Monden, Takushi, Tamaki, Yasuhiro, Morimoto, Hideki, Takeda, Tsutomu, Kobayashi, Tetsuro, Shimano, Takashi, Murakami, Hiroki, Mori, Takesada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918647/
https://www.ncbi.nlm.nih.gov/pubmed/1544869
http://dx.doi.org/10.1111/j.1349-7006.1992.tb02346.x
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author Yagyu, Toshio
Monden, Takushi
Tamaki, Yasuhiro
Morimoto, Hideki
Takeda, Tsutomu
Kobayashi, Tetsuro
Shimano, Takashi
Murakami, Hiroki
Mori, Takesada
author_facet Yagyu, Toshio
Monden, Takushi
Tamaki, Yasuhiro
Morimoto, Hideki
Takeda, Tsutomu
Kobayashi, Tetsuro
Shimano, Takashi
Murakami, Hiroki
Mori, Takesada
author_sort Yagyu, Toshio
collection PubMed
description Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete ≧ 100 ng/ml human immunoglobulin. The efficiency was remarkably high as compared with that from patients without the local immunotherapy. Further immunohistological examination showed that 5 hybridomas secreted IgM which selectively reacted with colonic cancers. The results indicate that local immunotherapy could be an adjunctive tool for the generation of highly potent human hybridomas through augmenting the host's immunity.
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spelling pubmed-59186472018-05-11 Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients Yagyu, Toshio Monden, Takushi Tamaki, Yasuhiro Morimoto, Hideki Takeda, Tsutomu Kobayashi, Tetsuro Shimano, Takashi Murakami, Hiroki Mori, Takesada Jpn J Cancer Res Rapid Communication Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete ≧ 100 ng/ml human immunoglobulin. The efficiency was remarkably high as compared with that from patients without the local immunotherapy. Further immunohistological examination showed that 5 hybridomas secreted IgM which selectively reacted with colonic cancers. The results indicate that local immunotherapy could be an adjunctive tool for the generation of highly potent human hybridomas through augmenting the host's immunity. Blackwell Publishing Ltd 1992-01 /pmc/articles/PMC5918647/ /pubmed/1544869 http://dx.doi.org/10.1111/j.1349-7006.1992.tb02346.x Text en
spellingShingle Rapid Communication
Yagyu, Toshio
Monden, Takushi
Tamaki, Yasuhiro
Morimoto, Hideki
Takeda, Tsutomu
Kobayashi, Tetsuro
Shimano, Takashi
Murakami, Hiroki
Mori, Takesada
Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
title Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
title_full Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
title_fullStr Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
title_full_unstemmed Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
title_short Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
title_sort use of a local immunotherapy as an adjunctive tool for the generation of human monoclonal antibodies from regional lymph nodes of colonic cancer patients
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918647/
https://www.ncbi.nlm.nih.gov/pubmed/1544869
http://dx.doi.org/10.1111/j.1349-7006.1992.tb02346.x
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