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Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients
Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1992
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918647/ https://www.ncbi.nlm.nih.gov/pubmed/1544869 http://dx.doi.org/10.1111/j.1349-7006.1992.tb02346.x |
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author | Yagyu, Toshio Monden, Takushi Tamaki, Yasuhiro Morimoto, Hideki Takeda, Tsutomu Kobayashi, Tetsuro Shimano, Takashi Murakami, Hiroki Mori, Takesada |
author_facet | Yagyu, Toshio Monden, Takushi Tamaki, Yasuhiro Morimoto, Hideki Takeda, Tsutomu Kobayashi, Tetsuro Shimano, Takashi Murakami, Hiroki Mori, Takesada |
author_sort | Yagyu, Toshio |
collection | PubMed |
description | Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete ≧ 100 ng/ml human immunoglobulin. The efficiency was remarkably high as compared with that from patients without the local immunotherapy. Further immunohistological examination showed that 5 hybridomas secreted IgM which selectively reacted with colonic cancers. The results indicate that local immunotherapy could be an adjunctive tool for the generation of highly potent human hybridomas through augmenting the host's immunity. |
format | Online Article Text |
id | pubmed-5918647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59186472018-05-11 Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients Yagyu, Toshio Monden, Takushi Tamaki, Yasuhiro Morimoto, Hideki Takeda, Tsutomu Kobayashi, Tetsuro Shimano, Takashi Murakami, Hiroki Mori, Takesada Jpn J Cancer Res Rapid Communication Human hybridomas were generated through the fusion of the human B‐lymphoblastoid cell line HO‐323 with the regional lymph node lymphocytes of colonic cancer patients who had received a local immunotherapy. A total of 353 hybridomas were obtained from 4 patients and 116 of these were found to secrete ≧ 100 ng/ml human immunoglobulin. The efficiency was remarkably high as compared with that from patients without the local immunotherapy. Further immunohistological examination showed that 5 hybridomas secreted IgM which selectively reacted with colonic cancers. The results indicate that local immunotherapy could be an adjunctive tool for the generation of highly potent human hybridomas through augmenting the host's immunity. Blackwell Publishing Ltd 1992-01 /pmc/articles/PMC5918647/ /pubmed/1544869 http://dx.doi.org/10.1111/j.1349-7006.1992.tb02346.x Text en |
spellingShingle | Rapid Communication Yagyu, Toshio Monden, Takushi Tamaki, Yasuhiro Morimoto, Hideki Takeda, Tsutomu Kobayashi, Tetsuro Shimano, Takashi Murakami, Hiroki Mori, Takesada Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients |
title | Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients |
title_full | Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients |
title_fullStr | Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients |
title_full_unstemmed | Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients |
title_short | Use of a Local Immunotherapy as an Adjunctive Tool for the Generation of Human Monoclonal Antibodies from Regional Lymph Nodes of Colonic Cancer Patients |
title_sort | use of a local immunotherapy as an adjunctive tool for the generation of human monoclonal antibodies from regional lymph nodes of colonic cancer patients |
topic | Rapid Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918647/ https://www.ncbi.nlm.nih.gov/pubmed/1544869 http://dx.doi.org/10.1111/j.1349-7006.1992.tb02346.x |
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