Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been widely repurposed for genome editing(1–4). High-fidelity (SpCas9-HF1) and enhanced specificity (eSpCas9(1.1)) variants exhibit substantially reduced off-target cleavage in human cells, but the mechanism of target discr...

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Detalles Bibliográficos
Autores principales: Chen, Janice S., Dagdas, Yavuz S., Kleinstiver, Benjamin P., Welch, Moira M., Sousa, Alexander A., Harrington, Lucas B., Sternberg, Samuel H., Joung, J. Keith, Yildiz, Ahmet, Doudna, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918688/
https://www.ncbi.nlm.nih.gov/pubmed/28931002
http://dx.doi.org/10.1038/nature24268

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918688/
https://www.ncbi.nlm.nih.gov/pubmed/28931002
http://dx.doi.org/10.1038/nature24268

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