Genomic Organization of the Human WT1 Gene

We have analyzed the genomic structure of the human WT1 gene, one of the recessive oncogenes for Wilms’tumor at chromosome lip 13. By analyses of three cosmids covering the WT1 gene as well as products generated by polymerase chain reaction, cleavage sites for 10 restriction enzymes were mapped in a...

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Detalles Bibliográficos
Autores principales: Tadokoro, Keiko, Oki, Noriko, Fujii, Hanako, Ohshima, Akira, Inoue, Tadashi, Yamada, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918721/
https://www.ncbi.nlm.nih.gov/pubmed/1483934
http://dx.doi.org/10.1111/j.1349-7006.1992.tb02745.x
Descripción
Sumario:We have analyzed the genomic structure of the human WT1 gene, one of the recessive oncogenes for Wilms’tumor at chromosome lip 13. By analyses of three cosmids covering the WT1 gene as well as products generated by polymerase chain reaction, cleavage sites for 10 restriction enzymes were mapped in a region of about 80 kb, and the positions of 10 exons were defined. We also mapped two polymorphic sites for TaqI. Our genomic map will be useful to analyze DNA abnormalities sometimes found in the tumors, as well as loss of heterozygosity.

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