Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization

Fluorescent in situ hybridization (FISH) is a powerful technique for gene mapping and multi‐color FISH allows us to determine directly the order of two or more probes on both metaphase and interphase chromosomes. We report the physical ordering of three DNA markers by three‐color FISH using two fluo...

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Detalles Bibliográficos
Autores principales: Inazawa, Johji, Ariyama, Takeshi, Abe, Tatsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918729/
https://www.ncbi.nlm.nih.gov/pubmed/1483939
http://dx.doi.org/10.1111/j.1349-7006.1992.tb02753.x
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author Inazawa, Johji
Ariyama, Takeshi
Abe, Tatsuo
author_facet Inazawa, Johji
Ariyama, Takeshi
Abe, Tatsuo
author_sort Inazawa, Johji
collection PubMed
description Fluorescent in situ hybridization (FISH) is a powerful technique for gene mapping and multi‐color FISH allows us to determine directly the order of two or more probes on both metaphase and interphase chromosomes. We report the physical ordering of three DNA markers by three‐color FISH using two fluorochrome dyes, fluorescein isothiocyanate (FITC; green) and rhodamine (red). The third color was visualized as a pseudo‐color (yellow) generated by optical interference with FITC and rhodamine. Using this system we could rapidly determine the order of three polymorphic DNA markers located on the 3p23–p21.2 bands which span a tumor suppressor gene for renal cell carcinoma, lung carcinoma, and several other types of tumors.
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spelling pubmed-59187292018-05-11 Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization Inazawa, Johji Ariyama, Takeshi Abe, Tatsuo Jpn J Cancer Res Rapid Communication Fluorescent in situ hybridization (FISH) is a powerful technique for gene mapping and multi‐color FISH allows us to determine directly the order of two or more probes on both metaphase and interphase chromosomes. We report the physical ordering of three DNA markers by three‐color FISH using two fluorochrome dyes, fluorescein isothiocyanate (FITC; green) and rhodamine (red). The third color was visualized as a pseudo‐color (yellow) generated by optical interference with FITC and rhodamine. Using this system we could rapidly determine the order of three polymorphic DNA markers located on the 3p23–p21.2 bands which span a tumor suppressor gene for renal cell carcinoma, lung carcinoma, and several other types of tumors. Blackwell Publishing Ltd 1992-12 /pmc/articles/PMC5918729/ /pubmed/1483939 http://dx.doi.org/10.1111/j.1349-7006.1992.tb02753.x Text en
spellingShingle Rapid Communication
Inazawa, Johji
Ariyama, Takeshi
Abe, Tatsuo
Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization
title Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization
title_full Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization
title_fullStr Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization
title_full_unstemmed Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization
title_short Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three‐color Fluorescent in situ Hybridization
title_sort physical ordering of three polymorphic dna markers spanning the regions containing a tumor suppressor gene of renal cell carcinoma by three‐color fluorescent in situ hybridization
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918729/
https://www.ncbi.nlm.nih.gov/pubmed/1483939
http://dx.doi.org/10.1111/j.1349-7006.1992.tb02753.x
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