Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction

We have developed a sensitive reverse transcription (RT)‐polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr‐specific primer sets, mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the s...

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Detalles Bibliográficos
Autores principales: Sugawara, Isamu, Watanabe, Masatoshi, Masunaga, Atsuko, Itoyama, Shinji, Ueda, Kazumitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918777/
https://www.ncbi.nlm.nih.gov/pubmed/1555993
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00076.x
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author Sugawara, Isamu
Watanabe, Masatoshi
Masunaga, Atsuko
Itoyama, Shinji
Ueda, Kazumitsu
author_facet Sugawara, Isamu
Watanabe, Masatoshi
Masunaga, Atsuko
Itoyama, Shinji
Ueda, Kazumitsu
author_sort Sugawara, Isamu
collection PubMed
description We have developed a sensitive reverse transcription (RT)‐polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr‐specific primer sets, mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should he used for effective RT‐PCR.
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spelling pubmed-59187772018-05-11 Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction Sugawara, Isamu Watanabe, Masatoshi Masunaga, Atsuko Itoyama, Shinji Ueda, Kazumitsu Jpn J Cancer Res Article We have developed a sensitive reverse transcription (RT)‐polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr‐specific primer sets, mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should he used for effective RT‐PCR. Blackwell Publishing Ltd 1992-02 /pmc/articles/PMC5918777/ /pubmed/1555993 http://dx.doi.org/10.1111/j.1349-7006.1992.tb00076.x Text en
spellingShingle Article
Sugawara, Isamu
Watanabe, Masatoshi
Masunaga, Atsuko
Itoyama, Shinji
Ueda, Kazumitsu
Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
title Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
title_full Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
title_fullStr Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
title_full_unstemmed Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
title_short Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction
title_sort primer‐dependent amplification of mdr1 mrna by polymerase chain reaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918777/
https://www.ncbi.nlm.nih.gov/pubmed/1555993
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00076.x
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