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Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG

Thirteen kinds of 45‐mer or 30‐mer synthetic oligonucleotides with sequences randomly selected from the cDNA encoding three kinds of protein of Mycobacterium bovis BCG were tested for their antitumor activity in a murine tumor system. Six out of the 13 single‐stranded oligonucleotides which containe...

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Autores principales: Kataoka, Tetsuro, Yamamoto, Saburo, Yamamoto, Toshiko, Kuramoto, Etsuro, Kimura, Yoshimitsu, Yano, Osamu, Tokunaga, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918807/
https://www.ncbi.nlm.nih.gov/pubmed/1582885
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00094.x
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author Kataoka, Tetsuro
Yamamoto, Saburo
Yamamoto, Toshiko
Kuramoto, Etsuro
Kimura, Yoshimitsu
Yano, Osamu
Tokunaga, Tohru
author_facet Kataoka, Tetsuro
Yamamoto, Saburo
Yamamoto, Toshiko
Kuramoto, Etsuro
Kimura, Yoshimitsu
Yano, Osamu
Tokunaga, Tohru
author_sort Kataoka, Tetsuro
collection PubMed
description Thirteen kinds of 45‐mer or 30‐mer synthetic oligonucleotides with sequences randomly selected from the cDNA encoding three kinds of protein of Mycobacterium bovis BCG were tested for their antitumor activity in a murine tumor system. Six out of the 13 single‐stranded oligonucleotides which contained one or more hexameric palindromic sequences showed strong antitumor activity while the others without palindromic structure did not. Namely, repeated intralesional injections of 100 μg of the 6 oligonucleotides caused regression of the established tumor but the other 7 were ineffective. When tumor cells were mixed with 100 μg of an effective oligonucleotide and injected into mice, tumor growth was markedly suppressed. These results suggested that palindromic structure is essential for the antitumor activity of the synthetic oligonucleotides.
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spelling pubmed-59188072018-05-11 Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG Kataoka, Tetsuro Yamamoto, Saburo Yamamoto, Toshiko Kuramoto, Etsuro Kimura, Yoshimitsu Yano, Osamu Tokunaga, Tohru Jpn J Cancer Res Rapid Communication Thirteen kinds of 45‐mer or 30‐mer synthetic oligonucleotides with sequences randomly selected from the cDNA encoding three kinds of protein of Mycobacterium bovis BCG were tested for their antitumor activity in a murine tumor system. Six out of the 13 single‐stranded oligonucleotides which contained one or more hexameric palindromic sequences showed strong antitumor activity while the others without palindromic structure did not. Namely, repeated intralesional injections of 100 μg of the 6 oligonucleotides caused regression of the established tumor but the other 7 were ineffective. When tumor cells were mixed with 100 μg of an effective oligonucleotide and injected into mice, tumor growth was markedly suppressed. These results suggested that palindromic structure is essential for the antitumor activity of the synthetic oligonucleotides. Blackwell Publishing Ltd 1992-03 /pmc/articles/PMC5918807/ /pubmed/1582885 http://dx.doi.org/10.1111/j.1349-7006.1992.tb00094.x Text en
spellingShingle Rapid Communication
Kataoka, Tetsuro
Yamamoto, Saburo
Yamamoto, Toshiko
Kuramoto, Etsuro
Kimura, Yoshimitsu
Yano, Osamu
Tokunaga, Tohru
Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG
title Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG
title_full Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG
title_fullStr Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG
title_full_unstemmed Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG
title_short Antitumor Activity of Synthetic Oligonucleotides with Sequences from cDNA Encoding Proteins of Mycobacterium bovis BCG
title_sort antitumor activity of synthetic oligonucleotides with sequences from cdna encoding proteins of mycobacterium bovis bcg
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918807/
https://www.ncbi.nlm.nih.gov/pubmed/1582885
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00094.x
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