Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization

A consensus primer‐mediated polymerase chain reaction devised to amplify a short sequence in LI region (L1‐PCR) efficiently detected genital human papillomaviruses (HPVs) in clinical materials. Nucleotide sequencing of the amplified fragment showed that L1‐PCR is also applicable to hitherto unsequen...

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Detalles Bibliográficos
Autores principales: Iwamoto, Aikichi, Yoshikawa, Hiroyuki, Kitagawa, Kyoko, Igarashi, Hiroko, Kawana, Takashi, Yoshikura, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918827/
https://www.ncbi.nlm.nih.gov/pubmed/1324233
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00107.x
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author Iwamoto, Aikichi
Yoshikawa, Hiroyuki
Kitagawa, Kyoko
Igarashi, Hiroko
Kawana, Takashi
Yoshikura, Hiroshi
author_facet Iwamoto, Aikichi
Yoshikawa, Hiroyuki
Kitagawa, Kyoko
Igarashi, Hiroko
Kawana, Takashi
Yoshikura, Hiroshi
author_sort Iwamoto, Aikichi
collection PubMed
description A consensus primer‐mediated polymerase chain reaction devised to amplify a short sequence in LI region (L1‐PCR) efficiently detected genital human papillomaviruses (HPVs) in clinical materials. Nucleotide sequencing of the amplified fragment showed that L1‐PCR is also applicable to hitherto unsequenced HPVs. By comparing the amplified 210 nucleotides HPVs were classified into six groups, which are consistent with clinical pictures and a grouping based on cross‐hybridization under the stringent condition.
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spelling pubmed-59188272018-05-11 Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization Iwamoto, Aikichi Yoshikawa, Hiroyuki Kitagawa, Kyoko Igarashi, Hiroko Kawana, Takashi Yoshikura, Hiroshi Jpn J Cancer Res Rapid Communication A consensus primer‐mediated polymerase chain reaction devised to amplify a short sequence in LI region (L1‐PCR) efficiently detected genital human papillomaviruses (HPVs) in clinical materials. Nucleotide sequencing of the amplified fragment showed that L1‐PCR is also applicable to hitherto unsequenced HPVs. By comparing the amplified 210 nucleotides HPVs were classified into six groups, which are consistent with clinical pictures and a grouping based on cross‐hybridization under the stringent condition. Blackwell Publishing Ltd 1992-04 /pmc/articles/PMC5918827/ /pubmed/1324233 http://dx.doi.org/10.1111/j.1349-7006.1992.tb00107.x Text en
spellingShingle Rapid Communication
Iwamoto, Aikichi
Yoshikawa, Hiroyuki
Kitagawa, Kyoko
Igarashi, Hiroko
Kawana, Takashi
Yoshikura, Hiroshi
Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization
title Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization
title_full Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization
title_fullStr Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization
title_full_unstemmed Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization
title_short Short Sequence in LI Region of Human Papillomaviruses Correlates with Clinical Pictures and Grouping by Cross‐hybridization
title_sort short sequence in li region of human papillomaviruses correlates with clinical pictures and grouping by cross‐hybridization
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918827/
https://www.ncbi.nlm.nih.gov/pubmed/1324233
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00107.x
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