Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody

To develop an experimental model of adult T‐cell leukemia/lymphoma in small animals, severe combined immunodeficiency (SCID) mice treated with anti‐asialo GM‐1 antibody were inoculated with MT‐2 cells, a cell line transformed by the human T‐cell leukemia virus (HTLV–I). Three mice injected with 4 ×...

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Detalles Bibliográficos
Autores principales: Ishihara, Shiro, Tachibana, Nobuyoshi, Okayama, Akihiko, Murai, Koichi, Tsuda, Kazunori, Mueller, Nancy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918837/
https://www.ncbi.nlm.nih.gov/pubmed/1506264
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00108.x
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author Ishihara, Shiro
Tachibana, Nobuyoshi
Okayama, Akihiko
Murai, Koichi
Tsuda, Kazunori
Mueller, Nancy
author_facet Ishihara, Shiro
Tachibana, Nobuyoshi
Okayama, Akihiko
Murai, Koichi
Tsuda, Kazunori
Mueller, Nancy
author_sort Ishihara, Shiro
collection PubMed
description To develop an experimental model of adult T‐cell leukemia/lymphoma in small animals, severe combined immunodeficiency (SCID) mice treated with anti‐asialo GM‐1 antibody were inoculated with MT‐2 cells, a cell line transformed by the human T‐cell leukemia virus (HTLV–I). Three mice injected with 4 × 10(7) cells subcutaneously or intramuscularly developed tumors at or near inoculation sites. Immunofluorescent antibody (IFA) staining for HTLV–I structural protein, p19, revealed the specific antigen in the cytoplasm of most cells from tumors and the DNA signals of HTLV–I proviral DNA were also positive in cellular DNA by polymerase chain reaction assay with HTLV–I tax gene primers, SK43/SK44. The MT–2 cells did not invade in mouse organs.
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spelling pubmed-59188372018-05-11 Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody Ishihara, Shiro Tachibana, Nobuyoshi Okayama, Akihiko Murai, Koichi Tsuda, Kazunori Mueller, Nancy Jpn J Cancer Res Article To develop an experimental model of adult T‐cell leukemia/lymphoma in small animals, severe combined immunodeficiency (SCID) mice treated with anti‐asialo GM‐1 antibody were inoculated with MT‐2 cells, a cell line transformed by the human T‐cell leukemia virus (HTLV–I). Three mice injected with 4 × 10(7) cells subcutaneously or intramuscularly developed tumors at or near inoculation sites. Immunofluorescent antibody (IFA) staining for HTLV–I structural protein, p19, revealed the specific antigen in the cytoplasm of most cells from tumors and the DNA signals of HTLV–I proviral DNA were also positive in cellular DNA by polymerase chain reaction assay with HTLV–I tax gene primers, SK43/SK44. The MT–2 cells did not invade in mouse organs. Blackwell Publishing Ltd 1992-04 /pmc/articles/PMC5918837/ /pubmed/1506264 http://dx.doi.org/10.1111/j.1349-7006.1992.tb00108.x Text en
spellingShingle Article
Ishihara, Shiro
Tachibana, Nobuyoshi
Okayama, Akihiko
Murai, Koichi
Tsuda, Kazunori
Mueller, Nancy
Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody
title Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody
title_full Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody
title_fullStr Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody
title_full_unstemmed Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody
title_short Successful Graft of HTLV‐I‐transformed Human T‐Cells (MT‐2) in Severe Combined Immunodeficiency Mice Treated with Anti‐asialo GM‐1 Antibody
title_sort successful graft of htlv‐i‐transformed human t‐cells (mt‐2) in severe combined immunodeficiency mice treated with anti‐asialo gm‐1 antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918837/
https://www.ncbi.nlm.nih.gov/pubmed/1506264
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00108.x
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