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Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies
The lectin‐like protein analogous to bovine conglutinin was purified from human serum. The carbohydrate‐binding ability of conglutinin‐like protein was inhibited by D‐mannose, N‐acetylglucosamine and L‐fucose as well as by mannan‐containing oligosaccharides. By applying a lectin‐based ELISA system i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1992
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918846/ https://www.ncbi.nlm.nih.gov/pubmed/1618696 http://dx.doi.org/10.1111/j.1349-7006.1992.tb01950.x |
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author | Ushijima, Hiroshi Schröder, Heinz C. Poznanovic, Slobodan Gasić, Miroslav J. Matthes, Eckart Müller, Werner E. G. |
author_facet | Ushijima, Hiroshi Schröder, Heinz C. Poznanovic, Slobodan Gasić, Miroslav J. Matthes, Eckart Müller, Werner E. G. |
author_sort | Ushijima, Hiroshi |
collection | PubMed |
description | The lectin‐like protein analogous to bovine conglutinin was purified from human serum. The carbohydrate‐binding ability of conglutinin‐like protein was inhibited by D‐mannose, N‐acetylglucosamine and L‐fucose as well as by mannan‐containing oligosaccharides. By applying a lectin‐based ELISA system it was demonstrated that conglutinin‐like protein binds to human immunodeficiency virus‐1 (HIV‐1) glycoprotein 120 (gpl20) via its carbohydrate binding site. In vitro experiments with T‐lymphoblastoid CEM cells revealed that conglutinin‐like protein abolishes infection by HIV‐1; a 50% cytoprotective concentration of 23.9 μg/ml was measured. These findings demonstrate that human conglutinin‐like protein binds to HIV‐gp120 and inhibits, under the described in vitro conditions, CEM cell infection. |
format | Online Article Text |
id | pubmed-5918846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59188462018-05-11 Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies Ushijima, Hiroshi Schröder, Heinz C. Poznanovic, Slobodan Gasić, Miroslav J. Matthes, Eckart Müller, Werner E. G. Jpn J Cancer Res Article The lectin‐like protein analogous to bovine conglutinin was purified from human serum. The carbohydrate‐binding ability of conglutinin‐like protein was inhibited by D‐mannose, N‐acetylglucosamine and L‐fucose as well as by mannan‐containing oligosaccharides. By applying a lectin‐based ELISA system it was demonstrated that conglutinin‐like protein binds to human immunodeficiency virus‐1 (HIV‐1) glycoprotein 120 (gpl20) via its carbohydrate binding site. In vitro experiments with T‐lymphoblastoid CEM cells revealed that conglutinin‐like protein abolishes infection by HIV‐1; a 50% cytoprotective concentration of 23.9 μg/ml was measured. These findings demonstrate that human conglutinin‐like protein binds to HIV‐gp120 and inhibits, under the described in vitro conditions, CEM cell infection. Blackwell Publishing Ltd 1992-05 /pmc/articles/PMC5918846/ /pubmed/1618696 http://dx.doi.org/10.1111/j.1349-7006.1992.tb01950.x Text en |
spellingShingle | Article Ushijima, Hiroshi Schröder, Heinz C. Poznanovic, Slobodan Gasić, Miroslav J. Matthes, Eckart Müller, Werner E. G. Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies |
title | Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies |
title_full | Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies |
title_fullStr | Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies |
title_full_unstemmed | Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies |
title_short | Inhibition of Human Immunodeficiency Virus‐1 Infection by Human Conglutinin‐like Protein: In vitro Studies |
title_sort | inhibition of human immunodeficiency virus‐1 infection by human conglutinin‐like protein: in vitro studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918846/ https://www.ncbi.nlm.nih.gov/pubmed/1618696 http://dx.doi.org/10.1111/j.1349-7006.1992.tb01950.x |
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