Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera

The reverse transcriptase (RT) activity of a lysate of human immunodeficiency virus type 1 (HIV) was almost completely inactivated by incubation at 56°C for 20—‐30 min. The heat‐inactivation of RT in the virus lysate or purified RT was partially inhibited in the presence of some human sera or plasma...

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Detalles Bibliográficos
Autores principales: Nagumo, Toshiyuki, Takeuchi, Yasuhiro, Hoshino, Hiroo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918888/
https://www.ncbi.nlm.nih.gov/pubmed/1379577
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00131.x
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author Nagumo, Toshiyuki
Takeuchi, Yasuhiro
Hoshino, Hiroo
author_facet Nagumo, Toshiyuki
Takeuchi, Yasuhiro
Hoshino, Hiroo
author_sort Nagumo, Toshiyuki
collection PubMed
description The reverse transcriptase (RT) activity of a lysate of human immunodeficiency virus type 1 (HIV) was almost completely inactivated by incubation at 56°C for 20—‐30 min. The heat‐inactivation of RT in the virus lysate or purified RT was partially inhibited in the presence of some human sera or plasma containing antibodies against HIV. The IgG fraction purified from the seropositive sera was responsible for stabilization of RT upon heat inactivation. This is a new assay system for detection of antibodies against RT.
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spelling pubmed-59188882018-05-11 Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera Nagumo, Toshiyuki Takeuchi, Yasuhiro Hoshino, Hiroo Jpn J Cancer Res Article The reverse transcriptase (RT) activity of a lysate of human immunodeficiency virus type 1 (HIV) was almost completely inactivated by incubation at 56°C for 20—‐30 min. The heat‐inactivation of RT in the virus lysate or purified RT was partially inhibited in the presence of some human sera or plasma containing antibodies against HIV. The IgG fraction purified from the seropositive sera was responsible for stabilization of RT upon heat inactivation. This is a new assay system for detection of antibodies against RT. Blackwell Publishing Ltd 1992-06 /pmc/articles/PMC5918888/ /pubmed/1379577 http://dx.doi.org/10.1111/j.1349-7006.1992.tb00131.x Text en
spellingShingle Article
Nagumo, Toshiyuki
Takeuchi, Yasuhiro
Hoshino, Hiroo
Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera
title Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera
title_full Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera
title_fullStr Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera
title_full_unstemmed Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera
title_short Inhibition of Heat Inactivation of Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Seropositive Sera
title_sort inhibition of heat inactivation of reverse transcriptase of human immunodeficiency virus type 1 by seropositive sera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918888/
https://www.ncbi.nlm.nih.gov/pubmed/1379577
http://dx.doi.org/10.1111/j.1349-7006.1992.tb00131.x
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AT hoshinohiroo inhibitionofheatinactivationofreversetranscriptaseofhumanimmunodeficiencyvirustype1byseropositivesera

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