Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines

The cytotoxic activity of human recombinant tumor necrosis factor (rHuTNF) (from 0.01 to 10000 U/ml) was assayed on six human ovarian cancer cell lines and one human cervical carcinoma cell line using a crystal violet assay. rHuTNF was cytotoxic to four cell lines (A2780, A2774, SW626, PAD, while 3...

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Detalles Bibliográficos
Autores principales: Russo, Patrizia, Parodi, Silvio, Billi, Giovanna, Oliva, Cristina, Venturing, Marco, Noviello, Elvira, Conte, PierFranco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918938/
https://www.ncbi.nlm.nih.gov/pubmed/1517145
http://dx.doi.org/10.1111/j.1349-7006.1992.tb01966.x
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author Russo, Patrizia
Parodi, Silvio
Billi, Giovanna
Oliva, Cristina
Venturing, Marco
Noviello, Elvira
Conte, PierFranco
author_facet Russo, Patrizia
Parodi, Silvio
Billi, Giovanna
Oliva, Cristina
Venturing, Marco
Noviello, Elvira
Conte, PierFranco
author_sort Russo, Patrizia
collection PubMed
description The cytotoxic activity of human recombinant tumor necrosis factor (rHuTNF) (from 0.01 to 10000 U/ml) was assayed on six human ovarian cancer cell lines and one human cervical carcinoma cell line using a crystal violet assay. rHuTNF was cytotoxic to four cell lines (A2780, A2774, SW626, PAD, while 3 cell lines (IGROV1, SKOV3, Mel80) were marginally sensitive to its activity. However, under the same experimental conditions rHuTNF markedly enhanced the cytotoxicity of mitoxantrone, a chemotherapeutic drug targeted at DNA topoisomerase II, in six cell lines. The potentiation of mitoxantrone cytotoxicity was not caused by increased drug accumulation after rHuTNF treatment. No significant increase in cytotoxicity to Me180 cell line was seen when rHuTNF was added to mitoxantrone.
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spelling pubmed-59189382018-05-11 Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines Russo, Patrizia Parodi, Silvio Billi, Giovanna Oliva, Cristina Venturing, Marco Noviello, Elvira Conte, PierFranco Jpn J Cancer Res Rapid Communication The cytotoxic activity of human recombinant tumor necrosis factor (rHuTNF) (from 0.01 to 10000 U/ml) was assayed on six human ovarian cancer cell lines and one human cervical carcinoma cell line using a crystal violet assay. rHuTNF was cytotoxic to four cell lines (A2780, A2774, SW626, PAD, while 3 cell lines (IGROV1, SKOV3, Mel80) were marginally sensitive to its activity. However, under the same experimental conditions rHuTNF markedly enhanced the cytotoxicity of mitoxantrone, a chemotherapeutic drug targeted at DNA topoisomerase II, in six cell lines. The potentiation of mitoxantrone cytotoxicity was not caused by increased drug accumulation after rHuTNF treatment. No significant increase in cytotoxicity to Me180 cell line was seen when rHuTNF was added to mitoxantrone. Blackwell Publishing Ltd 1992-07 /pmc/articles/PMC5918938/ /pubmed/1517145 http://dx.doi.org/10.1111/j.1349-7006.1992.tb01966.x Text en
spellingShingle Rapid Communication
Russo, Patrizia
Parodi, Silvio
Billi, Giovanna
Oliva, Cristina
Venturing, Marco
Noviello, Elvira
Conte, PierFranco
Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines
title Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines
title_full Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines
title_fullStr Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines
title_full_unstemmed Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines
title_short Potentiation by Tumor Necrosis Factor of Mitoxantrone Cytotoxicity to Human Ovarian Cancer Cell Lines
title_sort potentiation by tumor necrosis factor of mitoxantrone cytotoxicity to human ovarian cancer cell lines
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918938/
https://www.ncbi.nlm.nih.gov/pubmed/1517145
http://dx.doi.org/10.1111/j.1349-7006.1992.tb01966.x
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