Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product

A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3‐specific peptide (H(2)N‐(12)WRPTSAEGDFELGISSKQKRKKTKTVKMI(41)G‐COOH) and hybridizing the primed mouse splenic B cells with X63‐Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did...

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Detalles Bibliográficos
Autores principales: Sugawara, Isamu, Hirata, Akio, Ueda, Kazumitsu, Itoyama, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918957/
https://www.ncbi.nlm.nih.gov/pubmed/1328122
http://dx.doi.org/10.1111/j.1349-7006.1992.tb01982.x
Descripción
Sumario:A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3‐specific peptide (H(2)N‐(12)WRPTSAEGDFELGISSKQKRKKTKTVKMI(41)G‐COOH) and hybridizing the primed mouse splenic B cells with X63‐Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross‐react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues.

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