Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product

A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3‐specific peptide (H(2)N‐(12)WRPTSAEGDFELGISSKQKRKKTKTVKMI(41)G‐COOH) and hybridizing the primed mouse splenic B cells with X63‐Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did...

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Detalles Bibliográficos
Autores principales: Sugawara, Isamu, Hirata, Akio, Ueda, Kazumitsu, Itoyama, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918957/
https://www.ncbi.nlm.nih.gov/pubmed/1328122
http://dx.doi.org/10.1111/j.1349-7006.1992.tb01982.x
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author Sugawara, Isamu
Hirata, Akio
Ueda, Kazumitsu
Itoyama, Shinji
author_facet Sugawara, Isamu
Hirata, Akio
Ueda, Kazumitsu
Itoyama, Shinji
author_sort Sugawara, Isamu
collection PubMed
description A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3‐specific peptide (H(2)N‐(12)WRPTSAEGDFELGISSKQKRKKTKTVKMI(41)G‐COOH) and hybridizing the primed mouse splenic B cells with X63‐Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross‐react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues.
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spelling pubmed-59189572018-05-11 Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product Sugawara, Isamu Hirata, Akio Ueda, Kazumitsu Itoyama, Shinji Jpn J Cancer Res Rapid Communication A murine monoclonal antibody (MDR3M) (isotype: IgM) reactive with mdr3 gene product was generated by immunizing mice with mdr3‐specific peptide (H(2)N‐(12)WRPTSAEGDFELGISSKQKRKKTKTVKMI(41)G‐COOH) and hybridizing the primed mouse splenic B cells with X63‐Ag8,6.5.3 mouse plasmacytoma cells. MDR3M did not cross‐react with mdr1 gene product. This monoclonal antibody may be useful for analyzing the role of mdr3 gene product in cells and tissues. Blackwell Publishing Ltd 1992-08 /pmc/articles/PMC5918957/ /pubmed/1328122 http://dx.doi.org/10.1111/j.1349-7006.1992.tb01982.x Text en
spellingShingle Rapid Communication
Sugawara, Isamu
Hirata, Akio
Ueda, Kazumitsu
Itoyama, Shinji
Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product
title Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product
title_full Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product
title_fullStr Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product
title_full_unstemmed Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product
title_short Preparation and Characterization of a Murine Monoclonal Antibody (MDR3M) Reactive with mdr3 Gene Product
title_sort preparation and characterization of a murine monoclonal antibody (mdr3m) reactive with mdr3 gene product
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918957/
https://www.ncbi.nlm.nih.gov/pubmed/1328122
http://dx.doi.org/10.1111/j.1349-7006.1992.tb01982.x
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