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New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum

A new human hepatocellular carcinoma cell (HCC) line, designated SUHC‐1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV‐RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morpholo...

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Autores principales: Yoshizawa, Kaname, Kiyosawa, Kendo, Usuda, Seiichi, Yabu, Koji, Nakatsuji, Yoshiyuki, Yamada, Shuhei, Furuta, Kiyoshi, Tanaka, Eiji, Sodeyama, Takeshi, Furuta, Seiichi, Kaneko, Shuichi, Nakane, Paul K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918961/
https://www.ncbi.nlm.nih.gov/pubmed/1328123
http://dx.doi.org/10.1111/j.1349-7006.1992.tb01993.x
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author Yoshizawa, Kaname
Kiyosawa, Kendo
Usuda, Seiichi
Yabu, Koji
Nakatsuji, Yoshiyuki
Yamada, Shuhei
Furuta, Kiyoshi
Tanaka, Eiji
Sodeyama, Takeshi
Furuta, Seiichi
Kaneko, Shuichi
Nakane, Paul K.
author_facet Yoshizawa, Kaname
Kiyosawa, Kendo
Usuda, Seiichi
Yabu, Koji
Nakatsuji, Yoshiyuki
Yamada, Shuhei
Furuta, Kiyoshi
Tanaka, Eiji
Sodeyama, Takeshi
Furuta, Seiichi
Kaneko, Shuichi
Nakane, Paul K.
author_sort Yoshizawa, Kaname
collection PubMed
description A new human hepatocellular carcinoma cell (HCC) line, designated SUHC‐1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV‐RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase‐contrast and electron microscopy. The SUHC‐1 cells produced albumin and α(2)‐macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p–, 1q–, i(1q), i(17q) and 20q +) with a modal number of 91. HCV‐RNA was not detected in the supernatant of SUHC‐1 cells by nested polymerase chain reaction assay or in the SUHC‐1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC‐1 cell line. The SUHC‐1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.
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spelling pubmed-59189612018-05-11 New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum Yoshizawa, Kaname Kiyosawa, Kendo Usuda, Seiichi Yabu, Koji Nakatsuji, Yoshiyuki Yamada, Shuhei Furuta, Kiyoshi Tanaka, Eiji Sodeyama, Takeshi Furuta, Seiichi Kaneko, Shuichi Nakane, Paul K. Jpn J Cancer Res Article A new human hepatocellular carcinoma cell (HCC) line, designated SUHC‐1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV‐RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase‐contrast and electron microscopy. The SUHC‐1 cells produced albumin and α(2)‐macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p–, 1q–, i(1q), i(17q) and 20q +) with a modal number of 91. HCV‐RNA was not detected in the supernatant of SUHC‐1 cells by nested polymerase chain reaction assay or in the SUHC‐1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC‐1 cell line. The SUHC‐1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV. Blackwell Publishing Ltd 1992-08 /pmc/articles/PMC5918961/ /pubmed/1328123 http://dx.doi.org/10.1111/j.1349-7006.1992.tb01993.x Text en
spellingShingle Article
Yoshizawa, Kaname
Kiyosawa, Kendo
Usuda, Seiichi
Yabu, Koji
Nakatsuji, Yoshiyuki
Yamada, Shuhei
Furuta, Kiyoshi
Tanaka, Eiji
Sodeyama, Takeshi
Furuta, Seiichi
Kaneko, Shuichi
Nakane, Paul K.
New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum
title New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum
title_full New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum
title_fullStr New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum
title_full_unstemmed New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum
title_short New Hepatocellular Carcinoma Cell Line SUHC‐1 Established from a Patient with Hepatitis C Virus RNA in Serum
title_sort new hepatocellular carcinoma cell line suhc‐1 established from a patient with hepatitis c virus rna in serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918961/
https://www.ncbi.nlm.nih.gov/pubmed/1328123
http://dx.doi.org/10.1111/j.1349-7006.1992.tb01993.x
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