Functional Analysis of Mononuclear Cells Infiltrating into Tumors: Establishment of T Cell Hybridomas Exhibiting Distinct Interacting Abilities with Endothelial Cells and Extracellular Matrix Components

We have established eleven T cell hybridoma cell lines to investigate mechanisms controlling interaction of T lymphocytes with endothelial cells as well as extracellular matrix (ECM) proteins at the clonal level. T cell hybridomas were characterized and subdivided Into four groups on the basis of their interaction behavior with high endothelial venules (HEV). Group 1 (G1) exhibited strong adhesiveness. The binding was temperature‐ and divalent cation‐dependent. Group 2 exhibited both adhesiveness and transendothelial migration (TEM, i.e., transmigration beneath the cytoplasm of endothelial cells). Group 3 exhibited strong TEM. G2 and G3 hybridomas exhibited temperature‐independent and divalent cation‐independent binding to HEV. Group 4 exhibited nonspecific adhesiveness to the surface of a slide glass. BW 5147, a parent of T cell hybridomas, was classified as G4. TEM was dependent on both the nature of T cell hybridomas and endothelial cells. TEM was completely temperature‐dependent. TEM of G3 hybridomas was not divalent cation‐dependent. Each group of T cell hybridomas interacted with various ECM components.