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Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water

Establishment of an animal model of stomach carcinogenesis in mice was attempted using N‐methyl‐N‐nitrosourea (MNU) in the drinking water. One hundred and forty‐eight male 6‐week‐old C3H mice were given MNU in their drinking water at a concentration of 120 ppm (group 1), 60 ppm (group 2), 30 ppm (gr...

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Detalles Bibliográficos
Autores principales: Tatematsu, Masae, Yamamoto, Masami, Iwata, Hitoshi, Fukami, Hiroko, Yuasa, Hirofumi, Tezuka, Noriaki, Masui, Tsuneo, Nakanishi, Hayao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919110/
https://www.ncbi.nlm.nih.gov/pubmed/8294216
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02831.x
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author Tatematsu, Masae
Yamamoto, Masami
Iwata, Hitoshi
Fukami, Hiroko
Yuasa, Hirofumi
Tezuka, Noriaki
Masui, Tsuneo
Nakanishi, Hayao
author_facet Tatematsu, Masae
Yamamoto, Masami
Iwata, Hitoshi
Fukami, Hiroko
Yuasa, Hirofumi
Tezuka, Noriaki
Masui, Tsuneo
Nakanishi, Hayao
author_sort Tatematsu, Masae
collection PubMed
description Establishment of an animal model of stomach carcinogenesis in mice was attempted using N‐methyl‐N‐nitrosourea (MNU) in the drinking water. One hundred and forty‐eight male 6‐week‐old C3H mice were given MNU in their drinking water at a concentration of 120 ppm (group 1), 60 ppm (group 2), 30 ppm (group 3) or 0 ppm (group 4) for 30 weeks. At the end of this time, dose‐related induction of adenomatous hyperplasias was found. From weeks 31 to 54 adenocarcinomas developed in a dose‐dependent manner in groups 1, 2 and 3. In total, 6 well differentiated and 5 poorly differentiated adenocarcinomas as well as 6 signet ring cell carcinomas arose in 15 stomach cancer‐bearing animals in group 1, 4 well differentiated and 2 poorly differentiated adenocarcinomas with one signet ring cell carcinoma in 5 mice of group 2 and one well differentiated adenocarcinoma in group 3. In the forestomach, only one squamous cell carcinoma was found at week 54 in group 1 along with a single well differentiated adenocarcinoma in the duodenum. Thus, MNU in the drinking water selectively induced neoplastic lesions in the glandular stomach epithelium of mice.
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spelling pubmed-59191102018-05-11 Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water Tatematsu, Masae Yamamoto, Masami Iwata, Hitoshi Fukami, Hiroko Yuasa, Hirofumi Tezuka, Noriaki Masui, Tsuneo Nakanishi, Hayao Jpn J Cancer Res Article Establishment of an animal model of stomach carcinogenesis in mice was attempted using N‐methyl‐N‐nitrosourea (MNU) in the drinking water. One hundred and forty‐eight male 6‐week‐old C3H mice were given MNU in their drinking water at a concentration of 120 ppm (group 1), 60 ppm (group 2), 30 ppm (group 3) or 0 ppm (group 4) for 30 weeks. At the end of this time, dose‐related induction of adenomatous hyperplasias was found. From weeks 31 to 54 adenocarcinomas developed in a dose‐dependent manner in groups 1, 2 and 3. In total, 6 well differentiated and 5 poorly differentiated adenocarcinomas as well as 6 signet ring cell carcinomas arose in 15 stomach cancer‐bearing animals in group 1, 4 well differentiated and 2 poorly differentiated adenocarcinomas with one signet ring cell carcinoma in 5 mice of group 2 and one well differentiated adenocarcinoma in group 3. In the forestomach, only one squamous cell carcinoma was found at week 54 in group 1 along with a single well differentiated adenocarcinoma in the duodenum. Thus, MNU in the drinking water selectively induced neoplastic lesions in the glandular stomach epithelium of mice. Blackwell Publishing Ltd 1993-12 /pmc/articles/PMC5919110/ /pubmed/8294216 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02831.x Text en
spellingShingle Article
Tatematsu, Masae
Yamamoto, Masami
Iwata, Hitoshi
Fukami, Hiroko
Yuasa, Hirofumi
Tezuka, Noriaki
Masui, Tsuneo
Nakanishi, Hayao
Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water
title Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water
title_full Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water
title_fullStr Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water
title_full_unstemmed Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water
title_short Induction of Glandular Stomach Cancers in C3H Mice Treated with N‐Methyl‐N‐nitrosourea in the Drinking Water
title_sort induction of glandular stomach cancers in c3h mice treated with n‐methyl‐n‐nitrosourea in the drinking water
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919110/
https://www.ncbi.nlm.nih.gov/pubmed/8294216
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02831.x
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