Cargando…

Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats

Preneoplastic and neoplastic liver cell lesions, induced by EHEN (N‐ethyl‐N‐hydroxyethylnitrosamine) in rats, were investigated to establish the numbers of simultaneously expressed altered enzyme phenotypes within the lesion cells. The lesions were divided into 5 classes on the basis of altered expr...

Descripción completa

Detalles Bibliográficos
Autores principales: Yamaguchi, Shuji, Hakoi, Kazuo, Ozaki, Keisuke, Kato, Toshio, Tiwawech, Danai, Nagao, Shizuko, Takahashi, Hisahide, Matsumoto, Kazuyuki, Tsuda, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919116/
https://www.ncbi.nlm.nih.gov/pubmed/7904986
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02828.x
_version_ 1783317566604705792
author Yamaguchi, Shuji
Hakoi, Kazuo
Ozaki, Keisuke
Kato, Toshio
Tiwawech, Danai
Nagao, Shizuko
Takahashi, Hisahide
Matsumoto, Kazuyuki
Tsuda, Hiroyuki
author_facet Yamaguchi, Shuji
Hakoi, Kazuo
Ozaki, Keisuke
Kato, Toshio
Tiwawech, Danai
Nagao, Shizuko
Takahashi, Hisahide
Matsumoto, Kazuyuki
Tsuda, Hiroyuki
author_sort Yamaguchi, Shuji
collection PubMed
description Preneoplastic and neoplastic liver cell lesions, induced by EHEN (N‐ethyl‐N‐hydroxyethylnitrosamine) in rats, were investigated to establish the numbers of simultaneously expressed altered enzyme phenotypes within the lesion cells. The lesions were divided into 5 classes on the basis of altered expression in one or more of the following 5 enzymes: glutathione S‐transferase placental form, glucose‐6‐phosphate dehydrogenase, glucose‐6‐phosphatase, adenosine triphosphatase, and γ‐glutamyl transpeptidase. Class 1 lesions contained cells expressing one altered enzyme. Similarly, class 2, 3, 4 and 5 lesions had cells simultaneously expressing 2, 3, 4, and 5 enzyme alterations, respectively. Four histopathological categories of lesions, ACF (altered cell foci) (274 lesions), HN (hyperplastic nodules) (47 lesions), HCC (hepatocellular carcinomas) (99 lesions) and THC (transplanted hepatocellular carcinomas) (5 lesions) were studied. Proliferation potential was assessed in terms of 5‐bromo‐2′‐deoxyuridine (BrdU) incorporation. The distribution profiles of classes 1 to 5 showed a clear reciprocal change from low class (1 to 2 enzymes) predominance in ACF to high class (4 to 5 enzymes) predominance in HN. Increase of BrdU labeling indices was clearly correlated with progression from HN to HCC. Only a small population of class 5 ACF showed a high BrdU labeling index, indicating particular potential for further development. Thus, the stages of EHEN‐induced neoplasia were found to be characterized by gradual increase in the number of altered enzyme phenotypes, with acquisition of proliferative potential being associated with further progression towards malignant conversion.
format Online
Article
Text
id pubmed-5919116
institution National Center for Biotechnology Information
language English
publishDate 1993
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59191162018-05-11 Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats Yamaguchi, Shuji Hakoi, Kazuo Ozaki, Keisuke Kato, Toshio Tiwawech, Danai Nagao, Shizuko Takahashi, Hisahide Matsumoto, Kazuyuki Tsuda, Hiroyuki Jpn J Cancer Res Article Preneoplastic and neoplastic liver cell lesions, induced by EHEN (N‐ethyl‐N‐hydroxyethylnitrosamine) in rats, were investigated to establish the numbers of simultaneously expressed altered enzyme phenotypes within the lesion cells. The lesions were divided into 5 classes on the basis of altered expression in one or more of the following 5 enzymes: glutathione S‐transferase placental form, glucose‐6‐phosphate dehydrogenase, glucose‐6‐phosphatase, adenosine triphosphatase, and γ‐glutamyl transpeptidase. Class 1 lesions contained cells expressing one altered enzyme. Similarly, class 2, 3, 4 and 5 lesions had cells simultaneously expressing 2, 3, 4, and 5 enzyme alterations, respectively. Four histopathological categories of lesions, ACF (altered cell foci) (274 lesions), HN (hyperplastic nodules) (47 lesions), HCC (hepatocellular carcinomas) (99 lesions) and THC (transplanted hepatocellular carcinomas) (5 lesions) were studied. Proliferation potential was assessed in terms of 5‐bromo‐2′‐deoxyuridine (BrdU) incorporation. The distribution profiles of classes 1 to 5 showed a clear reciprocal change from low class (1 to 2 enzymes) predominance in ACF to high class (4 to 5 enzymes) predominance in HN. Increase of BrdU labeling indices was clearly correlated with progression from HN to HCC. Only a small population of class 5 ACF showed a high BrdU labeling index, indicating particular potential for further development. Thus, the stages of EHEN‐induced neoplasia were found to be characterized by gradual increase in the number of altered enzyme phenotypes, with acquisition of proliferative potential being associated with further progression towards malignant conversion. Blackwell Publishing Ltd 1993-12 /pmc/articles/PMC5919116/ /pubmed/7904986 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02828.x Text en
spellingShingle Article
Yamaguchi, Shuji
Hakoi, Kazuo
Ozaki, Keisuke
Kato, Toshio
Tiwawech, Danai
Nagao, Shizuko
Takahashi, Hisahide
Matsumoto, Kazuyuki
Tsuda, Hiroyuki
Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_full Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_fullStr Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_full_unstemmed Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_short Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_sort number of simultaneously expressed enzyme alterations correlates with progression of n‐ethyl‐n‐hydroxyethylnitrosamine‐induced hepatocarcinogenesis in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919116/
https://www.ncbi.nlm.nih.gov/pubmed/7904986
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02828.x
work_keys_str_mv AT yamaguchishuji numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT hakoikazuo numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT ozakikeisuke numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT katotoshio numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT tiwawechdanai numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT nagaoshizuko numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT takahashihisahide numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT matsumotokazuyuki numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats
AT tsudahiroyuki numberofsimultaneouslyexpressedenzymealterationscorrelateswithprogressionofnethylnhydroxyethylnitrosamineinducedhepatocarcinogenesisinrats