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Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats
Preneoplastic and neoplastic liver cell lesions, induced by EHEN (N‐ethyl‐N‐hydroxyethylnitrosamine) in rats, were investigated to establish the numbers of simultaneously expressed altered enzyme phenotypes within the lesion cells. The lesions were divided into 5 classes on the basis of altered expr...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1993
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919116/ https://www.ncbi.nlm.nih.gov/pubmed/7904986 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02828.x |
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author | Yamaguchi, Shuji Hakoi, Kazuo Ozaki, Keisuke Kato, Toshio Tiwawech, Danai Nagao, Shizuko Takahashi, Hisahide Matsumoto, Kazuyuki Tsuda, Hiroyuki |
author_facet | Yamaguchi, Shuji Hakoi, Kazuo Ozaki, Keisuke Kato, Toshio Tiwawech, Danai Nagao, Shizuko Takahashi, Hisahide Matsumoto, Kazuyuki Tsuda, Hiroyuki |
author_sort | Yamaguchi, Shuji |
collection | PubMed |
description | Preneoplastic and neoplastic liver cell lesions, induced by EHEN (N‐ethyl‐N‐hydroxyethylnitrosamine) in rats, were investigated to establish the numbers of simultaneously expressed altered enzyme phenotypes within the lesion cells. The lesions were divided into 5 classes on the basis of altered expression in one or more of the following 5 enzymes: glutathione S‐transferase placental form, glucose‐6‐phosphate dehydrogenase, glucose‐6‐phosphatase, adenosine triphosphatase, and γ‐glutamyl transpeptidase. Class 1 lesions contained cells expressing one altered enzyme. Similarly, class 2, 3, 4 and 5 lesions had cells simultaneously expressing 2, 3, 4, and 5 enzyme alterations, respectively. Four histopathological categories of lesions, ACF (altered cell foci) (274 lesions), HN (hyperplastic nodules) (47 lesions), HCC (hepatocellular carcinomas) (99 lesions) and THC (transplanted hepatocellular carcinomas) (5 lesions) were studied. Proliferation potential was assessed in terms of 5‐bromo‐2′‐deoxyuridine (BrdU) incorporation. The distribution profiles of classes 1 to 5 showed a clear reciprocal change from low class (1 to 2 enzymes) predominance in ACF to high class (4 to 5 enzymes) predominance in HN. Increase of BrdU labeling indices was clearly correlated with progression from HN to HCC. Only a small population of class 5 ACF showed a high BrdU labeling index, indicating particular potential for further development. Thus, the stages of EHEN‐induced neoplasia were found to be characterized by gradual increase in the number of altered enzyme phenotypes, with acquisition of proliferative potential being associated with further progression towards malignant conversion. |
format | Online Article Text |
id | pubmed-5919116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59191162018-05-11 Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats Yamaguchi, Shuji Hakoi, Kazuo Ozaki, Keisuke Kato, Toshio Tiwawech, Danai Nagao, Shizuko Takahashi, Hisahide Matsumoto, Kazuyuki Tsuda, Hiroyuki Jpn J Cancer Res Article Preneoplastic and neoplastic liver cell lesions, induced by EHEN (N‐ethyl‐N‐hydroxyethylnitrosamine) in rats, were investigated to establish the numbers of simultaneously expressed altered enzyme phenotypes within the lesion cells. The lesions were divided into 5 classes on the basis of altered expression in one or more of the following 5 enzymes: glutathione S‐transferase placental form, glucose‐6‐phosphate dehydrogenase, glucose‐6‐phosphatase, adenosine triphosphatase, and γ‐glutamyl transpeptidase. Class 1 lesions contained cells expressing one altered enzyme. Similarly, class 2, 3, 4 and 5 lesions had cells simultaneously expressing 2, 3, 4, and 5 enzyme alterations, respectively. Four histopathological categories of lesions, ACF (altered cell foci) (274 lesions), HN (hyperplastic nodules) (47 lesions), HCC (hepatocellular carcinomas) (99 lesions) and THC (transplanted hepatocellular carcinomas) (5 lesions) were studied. Proliferation potential was assessed in terms of 5‐bromo‐2′‐deoxyuridine (BrdU) incorporation. The distribution profiles of classes 1 to 5 showed a clear reciprocal change from low class (1 to 2 enzymes) predominance in ACF to high class (4 to 5 enzymes) predominance in HN. Increase of BrdU labeling indices was clearly correlated with progression from HN to HCC. Only a small population of class 5 ACF showed a high BrdU labeling index, indicating particular potential for further development. Thus, the stages of EHEN‐induced neoplasia were found to be characterized by gradual increase in the number of altered enzyme phenotypes, with acquisition of proliferative potential being associated with further progression towards malignant conversion. Blackwell Publishing Ltd 1993-12 /pmc/articles/PMC5919116/ /pubmed/7904986 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02828.x Text en |
spellingShingle | Article Yamaguchi, Shuji Hakoi, Kazuo Ozaki, Keisuke Kato, Toshio Tiwawech, Danai Nagao, Shizuko Takahashi, Hisahide Matsumoto, Kazuyuki Tsuda, Hiroyuki Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats |
title | Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats |
title_full | Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats |
title_fullStr | Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats |
title_full_unstemmed | Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats |
title_short | Number of Simultaneously Expressed Enzyme Alterations Correlates with Progression of N‐Ethyl‐N‐hydroxyethylnitrosamine‐induced Hepatocarcinogenesis in Rats |
title_sort | number of simultaneously expressed enzyme alterations correlates with progression of n‐ethyl‐n‐hydroxyethylnitrosamine‐induced hepatocarcinogenesis in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919116/ https://www.ncbi.nlm.nih.gov/pubmed/7904986 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02828.x |
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