Infrequent ras Mutation in Human Stomach Cancers

Mutations of ras oncogenes in 37 human stomach cancers and 13 adenomas were investigated with regard to the histological phenotypes using polymerase chain reaction (PCR), allele‐specific oligonucleotide hybridization and/or direct sequencing of the PCR products. The ras mutation was found only in one case (2.7%), the histology of which was poorly differentiated adenocarcinoma. We found no mutation in stomach adenomas. The mutation consisted of a guanine‐to‐adenine transition in the first base of codon 13 of c‐Ki‐ras which replaced wild‐type glycine with serine, indicating that a putative glycine‐to‐aspartic acid change is not necessarily the critical event for c‐Ki‐ras gene activation in codon 13. These results further confirm the infrequency of ras mutation in stomach tumors and also suggest that ras mutations are not specific to the differentiated type of stomach cancer.