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A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen
Twelve human monoclonal antibodies (HuMAb) were established by the fusion of (mouse × human) heteromyeloma cells with B‐lymphoblastoid cells derived from the regional lymph nodes of three patients with squamous cell carcinoma of the lung. They were tested for reactivity to two kinds of proteins (pur...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1993
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919199/ https://www.ncbi.nlm.nih.gov/pubmed/8396567 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02041.x |
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author | Kudo, Toshio Kobayashi, Syunsuke Murakami, Kazuhiro Takano, Ryo Nose, Masato Sato, Nobuyuki Saeki, Hisaaki Saijyo, Susumu Hiyamuta, Syuichi Kadota, Akihiko Tachibana, Takehiko |
author_facet | Kudo, Toshio Kobayashi, Syunsuke Murakami, Kazuhiro Takano, Ryo Nose, Masato Sato, Nobuyuki Saeki, Hisaaki Saijyo, Susumu Hiyamuta, Syuichi Kadota, Akihiko Tachibana, Takehiko |
author_sort | Kudo, Toshio |
collection | PubMed |
description | Twelve human monoclonal antibodies (HuMAb) were established by the fusion of (mouse × human) heteromyeloma cells with B‐lymphoblastoid cells derived from the regional lymph nodes of three patients with squamous cell carcinoma of the lung. They were tested for reactivity to two kinds of proteins (purified protein derivatives and bovine serum albumin) by ELISA, Sq‐19 (squamous cell carcinoma) culture cells by indirect membrane immunofluorescence tests, and Sq‐19 tumor xenograft by immunohistological study. Among them, one HuMAb 904F (IgM, λ) was selected. In indirect membrane immunofluorescence tests, this 904F antibody reacted with various kinds of cell lines, e.g. lung cancer, esophageal cancer, endometrial cancer, and stomach cancer. It did not react with malignant hematopoietic and diploid fibroblast cell lines. Immunohistologically, it stained the tumor nests of squamous cell carcinoma, adenocarcinoma, and large cell carcinoma of the lung. It also stained those of esophagus and colon, but not those of small cell carcinoma of lung, or stomach. On frozen‐section specimens of normal tissues from various organs, it showed only limited areas of positive staining. Limited positive findings were observed at a reticular zone of the adrenal gland, at the esophagus as weak staining, and at islets of the pancreas as very weak staining. Western blotting analysis demonstrated that it recognized a 54 kDa trypsin‐sensitive molecule which is expressed on the surface of tumor cells. These results suggest the 904F monoclonal antibody detects a novel tumor‐associated antigen which is recognized by the human immune system. |
format | Online Article Text |
id | pubmed-5919199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59191992018-05-11 A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen Kudo, Toshio Kobayashi, Syunsuke Murakami, Kazuhiro Takano, Ryo Nose, Masato Sato, Nobuyuki Saeki, Hisaaki Saijyo, Susumu Hiyamuta, Syuichi Kadota, Akihiko Tachibana, Takehiko Jpn J Cancer Res Article Twelve human monoclonal antibodies (HuMAb) were established by the fusion of (mouse × human) heteromyeloma cells with B‐lymphoblastoid cells derived from the regional lymph nodes of three patients with squamous cell carcinoma of the lung. They were tested for reactivity to two kinds of proteins (purified protein derivatives and bovine serum albumin) by ELISA, Sq‐19 (squamous cell carcinoma) culture cells by indirect membrane immunofluorescence tests, and Sq‐19 tumor xenograft by immunohistological study. Among them, one HuMAb 904F (IgM, λ) was selected. In indirect membrane immunofluorescence tests, this 904F antibody reacted with various kinds of cell lines, e.g. lung cancer, esophageal cancer, endometrial cancer, and stomach cancer. It did not react with malignant hematopoietic and diploid fibroblast cell lines. Immunohistologically, it stained the tumor nests of squamous cell carcinoma, adenocarcinoma, and large cell carcinoma of the lung. It also stained those of esophagus and colon, but not those of small cell carcinoma of lung, or stomach. On frozen‐section specimens of normal tissues from various organs, it showed only limited areas of positive staining. Limited positive findings were observed at a reticular zone of the adrenal gland, at the esophagus as weak staining, and at islets of the pancreas as very weak staining. Western blotting analysis demonstrated that it recognized a 54 kDa trypsin‐sensitive molecule which is expressed on the surface of tumor cells. These results suggest the 904F monoclonal antibody detects a novel tumor‐associated antigen which is recognized by the human immune system. Blackwell Publishing Ltd 1993-07 /pmc/articles/PMC5919199/ /pubmed/8396567 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02041.x Text en |
spellingShingle | Article Kudo, Toshio Kobayashi, Syunsuke Murakami, Kazuhiro Takano, Ryo Nose, Masato Sato, Nobuyuki Saeki, Hisaaki Saijyo, Susumu Hiyamuta, Syuichi Kadota, Akihiko Tachibana, Takehiko A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen |
title | A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen |
title_full | A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen |
title_fullStr | A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen |
title_full_unstemmed | A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen |
title_short | A Novel Human Monoclonal Antibody Directed to a Tumor‐associated Antigen |
title_sort | novel human monoclonal antibody directed to a tumor‐associated antigen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919199/ https://www.ncbi.nlm.nih.gov/pubmed/8396567 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02041.x |
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