Cargando…

Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats

The effects of two aromatic thiocyanates, benzyl isothiocyanate (BITC) and benzyl thiocyanate (BTC), on diethylnitrosamine (DEN)‐induced hepatocarcinogenesis were examined in rats. A total of 108 male ACI/N rats, 5 weeks old, were divided into 6 groups (18 rats in each). Group 1 was given a single i...

Descripción completa

Detalles Bibliográficos
Autores principales: Sugie, Shigeyuki, Okumura, Ataru, Tanaka, Takuji, Mori, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919262/
https://www.ncbi.nlm.nih.gov/pubmed/8104919
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02059.x
_version_ 1783317586759385088
author Sugie, Shigeyuki
Okumura, Ataru
Tanaka, Takuji
Mori, Hideki
author_facet Sugie, Shigeyuki
Okumura, Ataru
Tanaka, Takuji
Mori, Hideki
author_sort Sugie, Shigeyuki
collection PubMed
description The effects of two aromatic thiocyanates, benzyl isothiocyanate (BITC) and benzyl thiocyanate (BTC), on diethylnitrosamine (DEN)‐induced hepatocarcinogenesis were examined in rats. A total of 108 male ACI/N rats, 5 weeks old, were divided into 6 groups (18 rats in each). Group 1 was given a single i.p, injection of DEN (200 mg/kg body weight) one week after the start of the experiment and then kept on the basal diet until the end of the experiment (1 year). Groups 2 and 3 were treated with DEN and received dietary BITC (100 ppm) or BTC (100 ppm), respectively, throughout the experimental duration. Groups 4 and 5 were not given the carcinogen and were fed the diet containing BITC or BTC, respectively. Group 6 was kept on the basal diet alone and served as a control. Liver neoplasms were seen in Groups 1, 2 and 3. Incidence and average number of liver neoplasms in Group 2 were significantly smaller than in Group 1 (P<0.0005 and P<0.001, respectively). The incidence of liver neoplasms in Group 3 was slightly lower than in Group 1, although the difference was not statistically significant. The numbers of glutathione S‐transferase placental form (GST‐P)‐positive foci in Group 2 and γ‐glutarnyltranspepridase (GGT)‐positive foci in Groups 2 and 3 were significantly smaller than those in Group 1 (P<0.001). The average and unit areas of GST‐P‐ or GGT‐positive foci in Group 2 or 3 were also significantly smaller than those in Group 1 (P<0.05). These results suggest that BITC and BTC are chemopreventive agents for DEN‐induced liver tumorigenesis.
format Online
Article
Text
id pubmed-5919262
institution National Center for Biotechnology Information
language English
publishDate 1993
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59192622018-05-11 Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats Sugie, Shigeyuki Okumura, Ataru Tanaka, Takuji Mori, Hideki Jpn J Cancer Res Article The effects of two aromatic thiocyanates, benzyl isothiocyanate (BITC) and benzyl thiocyanate (BTC), on diethylnitrosamine (DEN)‐induced hepatocarcinogenesis were examined in rats. A total of 108 male ACI/N rats, 5 weeks old, were divided into 6 groups (18 rats in each). Group 1 was given a single i.p, injection of DEN (200 mg/kg body weight) one week after the start of the experiment and then kept on the basal diet until the end of the experiment (1 year). Groups 2 and 3 were treated with DEN and received dietary BITC (100 ppm) or BTC (100 ppm), respectively, throughout the experimental duration. Groups 4 and 5 were not given the carcinogen and were fed the diet containing BITC or BTC, respectively. Group 6 was kept on the basal diet alone and served as a control. Liver neoplasms were seen in Groups 1, 2 and 3. Incidence and average number of liver neoplasms in Group 2 were significantly smaller than in Group 1 (P<0.0005 and P<0.001, respectively). The incidence of liver neoplasms in Group 3 was slightly lower than in Group 1, although the difference was not statistically significant. The numbers of glutathione S‐transferase placental form (GST‐P)‐positive foci in Group 2 and γ‐glutarnyltranspepridase (GGT)‐positive foci in Groups 2 and 3 were significantly smaller than those in Group 1 (P<0.001). The average and unit areas of GST‐P‐ or GGT‐positive foci in Group 2 or 3 were also significantly smaller than those in Group 1 (P<0.05). These results suggest that BITC and BTC are chemopreventive agents for DEN‐induced liver tumorigenesis. Blackwell Publishing Ltd 1993-08 /pmc/articles/PMC5919262/ /pubmed/8104919 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02059.x Text en
spellingShingle Article
Sugie, Shigeyuki
Okumura, Ataru
Tanaka, Takuji
Mori, Hideki
Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_full Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_fullStr Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_full_unstemmed Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_short Inhibitory Effects of Benzyl Isothiocyanate and Benzyl Thiocyanate on Diethylnitrosamine‐induced Hepatocarcinogenesis in Rats
title_sort inhibitory effects of benzyl isothiocyanate and benzyl thiocyanate on diethylnitrosamine‐induced hepatocarcinogenesis in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919262/
https://www.ncbi.nlm.nih.gov/pubmed/8104919
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02059.x
work_keys_str_mv AT sugieshigeyuki inhibitoryeffectsofbenzylisothiocyanateandbenzylthiocyanateondiethylnitrosamineinducedhepatocarcinogenesisinrats
AT okumuraataru inhibitoryeffectsofbenzylisothiocyanateandbenzylthiocyanateondiethylnitrosamineinducedhepatocarcinogenesisinrats
AT tanakatakuji inhibitoryeffectsofbenzylisothiocyanateandbenzylthiocyanateondiethylnitrosamineinducedhepatocarcinogenesisinrats
AT morihideki inhibitoryeffectsofbenzylisothiocyanateandbenzylthiocyanateondiethylnitrosamineinducedhepatocarcinogenesisinrats