Cargando…

Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas

The expression of mRNA for amphiregulin (AR), a novel gene of the epidermal growth factor family, was examined in 8 human gastric carcinoma cell lines and 32 gastric carcinoma tissues as well as corresponding normal mucosa. Of the 8 gastric carcinoma cell lines, 7 expressed 1.4 kb AR mRNA at various...

Descripción completa

Detalles Bibliográficos
Autores principales: Kitadai, Yasuhiko, Yasui, Wataru, Yokozaki, Hiroshi, Kuniyasu, Hiroki, Ayhan, Ayse, Haruma, Ken, Kajiyama, Goro, Johnson, Gibbes R., Tahara, Eiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919273/
https://www.ncbi.nlm.nih.gov/pubmed/8407551
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02061.x
_version_ 1783317589332590592
author Kitadai, Yasuhiko
Yasui, Wataru
Yokozaki, Hiroshi
Kuniyasu, Hiroki
Ayhan, Ayse
Haruma, Ken
Kajiyama, Goro
Johnson, Gibbes R.
Tahara, Eiichi
author_facet Kitadai, Yasuhiko
Yasui, Wataru
Yokozaki, Hiroshi
Kuniyasu, Hiroki
Ayhan, Ayse
Haruma, Ken
Kajiyama, Goro
Johnson, Gibbes R.
Tahara, Eiichi
author_sort Kitadai, Yasuhiko
collection PubMed
description The expression of mRNA for amphiregulin (AR), a novel gene of the epidermal growth factor family, was examined in 8 human gastric carcinoma cell lines and 32 gastric carcinoma tissues as well as corresponding normal mucosa. Of the 8 gastric carcinoma cell lines, 7 expressed 1.4 kb AR mRNA at various levels. The expression of AR mRNA by TMK‐1 and MKN‐28 cells was increased by treatment with epidermal growth factor or transforming growth factor α. In surgical cases, all the gastric carcinoma tissues and their adjacent normal mucosa expressed AR mRNA. Interestingly, 20 (62.5%) out of 32 tumors expressed AR mRNA at higher levels than their corresponding normal mucosas (tumor/normal ≥1.2). No obvious correlation was observed between the AR mRNA levels and the histological types or tumor staging of gastric carcinoma. Immunohistochemically, AR protein was localized to the cytoplasm and/or nucleus in tumor cells. These results suggest that AR produced by tumor cells may be involved in the pathogenesis and/or progression of human gastric carcinoma.
format Online
Article
Text
id pubmed-5919273
institution National Center for Biotechnology Information
language English
publishDate 1993
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-59192732018-05-11 Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas Kitadai, Yasuhiko Yasui, Wataru Yokozaki, Hiroshi Kuniyasu, Hiroki Ayhan, Ayse Haruma, Ken Kajiyama, Goro Johnson, Gibbes R. Tahara, Eiichi Jpn J Cancer Res Article The expression of mRNA for amphiregulin (AR), a novel gene of the epidermal growth factor family, was examined in 8 human gastric carcinoma cell lines and 32 gastric carcinoma tissues as well as corresponding normal mucosa. Of the 8 gastric carcinoma cell lines, 7 expressed 1.4 kb AR mRNA at various levels. The expression of AR mRNA by TMK‐1 and MKN‐28 cells was increased by treatment with epidermal growth factor or transforming growth factor α. In surgical cases, all the gastric carcinoma tissues and their adjacent normal mucosa expressed AR mRNA. Interestingly, 20 (62.5%) out of 32 tumors expressed AR mRNA at higher levels than their corresponding normal mucosas (tumor/normal ≥1.2). No obvious correlation was observed between the AR mRNA levels and the histological types or tumor staging of gastric carcinoma. Immunohistochemically, AR protein was localized to the cytoplasm and/or nucleus in tumor cells. These results suggest that AR produced by tumor cells may be involved in the pathogenesis and/or progression of human gastric carcinoma. Blackwell Publishing Ltd 1993-08 /pmc/articles/PMC5919273/ /pubmed/8407551 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02061.x Text en
spellingShingle Article
Kitadai, Yasuhiko
Yasui, Wataru
Yokozaki, Hiroshi
Kuniyasu, Hiroki
Ayhan, Ayse
Haruma, Ken
Kajiyama, Goro
Johnson, Gibbes R.
Tahara, Eiichi
Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas
title Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas
title_full Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas
title_fullStr Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas
title_full_unstemmed Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas
title_short Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas
title_sort expression of amphiregulin, a novel gene of the epidermal growth factor family, in human gastric carcinomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919273/
https://www.ncbi.nlm.nih.gov/pubmed/8407551
http://dx.doi.org/10.1111/j.1349-7006.1993.tb02061.x
work_keys_str_mv AT kitadaiyasuhiko expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT yasuiwataru expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT yokozakihiroshi expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT kuniyasuhiroki expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT ayhanayse expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT harumaken expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT kajiyamagoro expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT johnsongibbesr expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas
AT taharaeiichi expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas