Cargando…
Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas
The expression of mRNA for amphiregulin (AR), a novel gene of the epidermal growth factor family, was examined in 8 human gastric carcinoma cell lines and 32 gastric carcinoma tissues as well as corresponding normal mucosa. Of the 8 gastric carcinoma cell lines, 7 expressed 1.4 kb AR mRNA at various...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1993
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919273/ https://www.ncbi.nlm.nih.gov/pubmed/8407551 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02061.x |
_version_ | 1783317589332590592 |
---|---|
author | Kitadai, Yasuhiko Yasui, Wataru Yokozaki, Hiroshi Kuniyasu, Hiroki Ayhan, Ayse Haruma, Ken Kajiyama, Goro Johnson, Gibbes R. Tahara, Eiichi |
author_facet | Kitadai, Yasuhiko Yasui, Wataru Yokozaki, Hiroshi Kuniyasu, Hiroki Ayhan, Ayse Haruma, Ken Kajiyama, Goro Johnson, Gibbes R. Tahara, Eiichi |
author_sort | Kitadai, Yasuhiko |
collection | PubMed |
description | The expression of mRNA for amphiregulin (AR), a novel gene of the epidermal growth factor family, was examined in 8 human gastric carcinoma cell lines and 32 gastric carcinoma tissues as well as corresponding normal mucosa. Of the 8 gastric carcinoma cell lines, 7 expressed 1.4 kb AR mRNA at various levels. The expression of AR mRNA by TMK‐1 and MKN‐28 cells was increased by treatment with epidermal growth factor or transforming growth factor α. In surgical cases, all the gastric carcinoma tissues and their adjacent normal mucosa expressed AR mRNA. Interestingly, 20 (62.5%) out of 32 tumors expressed AR mRNA at higher levels than their corresponding normal mucosas (tumor/normal ≥1.2). No obvious correlation was observed between the AR mRNA levels and the histological types or tumor staging of gastric carcinoma. Immunohistochemically, AR protein was localized to the cytoplasm and/or nucleus in tumor cells. These results suggest that AR produced by tumor cells may be involved in the pathogenesis and/or progression of human gastric carcinoma. |
format | Online Article Text |
id | pubmed-5919273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59192732018-05-11 Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas Kitadai, Yasuhiko Yasui, Wataru Yokozaki, Hiroshi Kuniyasu, Hiroki Ayhan, Ayse Haruma, Ken Kajiyama, Goro Johnson, Gibbes R. Tahara, Eiichi Jpn J Cancer Res Article The expression of mRNA for amphiregulin (AR), a novel gene of the epidermal growth factor family, was examined in 8 human gastric carcinoma cell lines and 32 gastric carcinoma tissues as well as corresponding normal mucosa. Of the 8 gastric carcinoma cell lines, 7 expressed 1.4 kb AR mRNA at various levels. The expression of AR mRNA by TMK‐1 and MKN‐28 cells was increased by treatment with epidermal growth factor or transforming growth factor α. In surgical cases, all the gastric carcinoma tissues and their adjacent normal mucosa expressed AR mRNA. Interestingly, 20 (62.5%) out of 32 tumors expressed AR mRNA at higher levels than their corresponding normal mucosas (tumor/normal ≥1.2). No obvious correlation was observed between the AR mRNA levels and the histological types or tumor staging of gastric carcinoma. Immunohistochemically, AR protein was localized to the cytoplasm and/or nucleus in tumor cells. These results suggest that AR produced by tumor cells may be involved in the pathogenesis and/or progression of human gastric carcinoma. Blackwell Publishing Ltd 1993-08 /pmc/articles/PMC5919273/ /pubmed/8407551 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02061.x Text en |
spellingShingle | Article Kitadai, Yasuhiko Yasui, Wataru Yokozaki, Hiroshi Kuniyasu, Hiroki Ayhan, Ayse Haruma, Ken Kajiyama, Goro Johnson, Gibbes R. Tahara, Eiichi Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas |
title | Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas |
title_full | Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas |
title_fullStr | Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas |
title_full_unstemmed | Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas |
title_short | Expression of Amphiregulin, a Novel Gene of the Epidermal Growth Factor Family, in Human Gastric Carcinomas |
title_sort | expression of amphiregulin, a novel gene of the epidermal growth factor family, in human gastric carcinomas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919273/ https://www.ncbi.nlm.nih.gov/pubmed/8407551 http://dx.doi.org/10.1111/j.1349-7006.1993.tb02061.x |
work_keys_str_mv | AT kitadaiyasuhiko expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT yasuiwataru expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT yokozakihiroshi expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT kuniyasuhiroki expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT ayhanayse expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT harumaken expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT kajiyamagoro expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT johnsongibbesr expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas AT taharaeiichi expressionofamphiregulinanovelgeneoftheepidermalgrowthfactorfamilyinhumangastriccarcinomas |