High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes

Structural abnormality of T cell receptor (TCR)‐CD3 complex on the cell surface was investigated in peripheral blood lymphocytes (PBL) from 55 cancer patients. In 24 of the 68 tests done on these patients, the CD3C chain was not detected by immunoprecipitation with anti‐CD3ζ monoclonal antibody (mAb...

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Autores principales: Gunji, Yoshio, Hori, Seiji, Aoe, Tomohiko, Asano, Takehide, Ochiai, Takenori, Isono, Kaichi, Saito, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1994
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919393/
https://www.ncbi.nlm.nih.gov/pubmed/7852180
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02927.x
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author Gunji, Yoshio
Hori, Seiji
Aoe, Tomohiko
Asano, Takehide
Ochiai, Takenori
Isono, Kaichi
Saito, Takashi
author_facet Gunji, Yoshio
Hori, Seiji
Aoe, Tomohiko
Asano, Takehide
Ochiai, Takenori
Isono, Kaichi
Saito, Takashi
author_sort Gunji, Yoshio
collection PubMed
description Structural abnormality of T cell receptor (TCR)‐CD3 complex on the cell surface was investigated in peripheral blood lymphocytes (PBL) from 55 cancer patients. In 24 of the 68 tests done on these patients, the CD3C chain was not detected by immunoprecipitation with anti‐CD3ζ monoclonal antibody (mAb), but was observed with anti‐CD3ζ mAb, suggesting that a high frequency of cancer patients possesses abnormal T cell receptor (TCR) complex in PBL. On the other hand, the total ζ chain was missing in several advanced cases. During follow‐up of several patients, the ζ chain became undetectable after two or three months of cancer progression. It appears that immunosuppressive status can be monitored by analyzing the TCR‐CD3 complex on the cell surface of PBL.
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spelling pubmed-59193932018-05-11 High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes Gunji, Yoshio Hori, Seiji Aoe, Tomohiko Asano, Takehide Ochiai, Takenori Isono, Kaichi Saito, Takashi Jpn J Cancer Res Article Structural abnormality of T cell receptor (TCR)‐CD3 complex on the cell surface was investigated in peripheral blood lymphocytes (PBL) from 55 cancer patients. In 24 of the 68 tests done on these patients, the CD3C chain was not detected by immunoprecipitation with anti‐CD3ζ monoclonal antibody (mAb), but was observed with anti‐CD3ζ mAb, suggesting that a high frequency of cancer patients possesses abnormal T cell receptor (TCR) complex in PBL. On the other hand, the total ζ chain was missing in several advanced cases. During follow‐up of several patients, the ζ chain became undetectable after two or three months of cancer progression. It appears that immunosuppressive status can be monitored by analyzing the TCR‐CD3 complex on the cell surface of PBL. Blackwell Publishing Ltd 1994-12 /pmc/articles/PMC5919393/ /pubmed/7852180 http://dx.doi.org/10.1111/j.1349-7006.1994.tb02927.x Text en
spellingShingle Article
Gunji, Yoshio
Hori, Seiji
Aoe, Tomohiko
Asano, Takehide
Ochiai, Takenori
Isono, Kaichi
Saito, Takashi
High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes
title High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes
title_full High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes
title_fullStr High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes
title_full_unstemmed High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes
title_short High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor‐CD3 Complex in Peripheral Blood T Lymphocytes
title_sort high frequency of cancer patients with abnormal assembly of the t cell receptor‐cd3 complex in peripheral blood t lymphocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919393/
https://www.ncbi.nlm.nih.gov/pubmed/7852180
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02927.x
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