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Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine

Colon tumors were induced in F344 rats by three heterocyclic amines (HCAs), 2‐amino‐6‐methyl‐dipyrido[1,2‐a:3′, 2′‐d]imidazole (Glu‐P‐1), 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ) or 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP), and examined for p53 mutations. Seven carcinomas induced b...

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Detalles Bibliográficos
Autores principales: Makino, Hiroshi, Ushijima, Toshikazu, Kakiuchi, Hideki, Onda, Masahiko, Ito, Nobuyuki, Sugimura, Takashi, Nagao, Minako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919493/
https://www.ncbi.nlm.nih.gov/pubmed/8014110
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02388.x
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author Makino, Hiroshi
Ushijima, Toshikazu
Kakiuchi, Hideki
Onda, Masahiko
Ito, Nobuyuki
Sugimura, Takashi
Nagao, Minako
author_facet Makino, Hiroshi
Ushijima, Toshikazu
Kakiuchi, Hideki
Onda, Masahiko
Ito, Nobuyuki
Sugimura, Takashi
Nagao, Minako
author_sort Makino, Hiroshi
collection PubMed
description Colon tumors were induced in F344 rats by three heterocyclic amines (HCAs), 2‐amino‐6‐methyl‐dipyrido[1,2‐a:3′, 2′‐d]imidazole (Glu‐P‐1), 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ) or 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP), and examined for p53 mutations. Seven carcinomas induced by Glu‐P‐1, and nine carcinomas and two adenomas induced by IQ were examined by cDNA‐polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) analysis from codon 103 to 391 of p53, which encompasses the conserved regions II to IV. Nine carcinomas induced by PhIP were examined by genomic PCR‐SSCP analysis of exons 5 to 7 (from codon 124 to 304), which encompasses the 3’half of the conserved region II and all the conserved regions III‐V. No band shifts were found in any of these tumors under at least two conditions of SSCP analysis. Our previous study had shown a Ki‐ras mutation in only one Glu‐P‐1‐induced adenocarcinoma among the same 27 colon tumors, and no other mutation of ras family genes had been found. HCA‐induced rat colon tumors appear to represent a group of human colon tumors in which neither Ki‐ras nor p53 is involved.
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spelling pubmed-59194932018-05-11 Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine Makino, Hiroshi Ushijima, Toshikazu Kakiuchi, Hideki Onda, Masahiko Ito, Nobuyuki Sugimura, Takashi Nagao, Minako Jpn J Cancer Res Article Colon tumors were induced in F344 rats by three heterocyclic amines (HCAs), 2‐amino‐6‐methyl‐dipyrido[1,2‐a:3′, 2′‐d]imidazole (Glu‐P‐1), 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ) or 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP), and examined for p53 mutations. Seven carcinomas induced by Glu‐P‐1, and nine carcinomas and two adenomas induced by IQ were examined by cDNA‐polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) analysis from codon 103 to 391 of p53, which encompasses the conserved regions II to IV. Nine carcinomas induced by PhIP were examined by genomic PCR‐SSCP analysis of exons 5 to 7 (from codon 124 to 304), which encompasses the 3’half of the conserved region II and all the conserved regions III‐V. No band shifts were found in any of these tumors under at least two conditions of SSCP analysis. Our previous study had shown a Ki‐ras mutation in only one Glu‐P‐1‐induced adenocarcinoma among the same 27 colon tumors, and no other mutation of ras family genes had been found. HCA‐induced rat colon tumors appear to represent a group of human colon tumors in which neither Ki‐ras nor p53 is involved. Blackwell Publishing Ltd 1994-05 /pmc/articles/PMC5919493/ /pubmed/8014110 http://dx.doi.org/10.1111/j.1349-7006.1994.tb02388.x Text en
spellingShingle Article
Makino, Hiroshi
Ushijima, Toshikazu
Kakiuchi, Hideki
Onda, Masahiko
Ito, Nobuyuki
Sugimura, Takashi
Nagao, Minako
Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine
title Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine
title_full Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine
title_fullStr Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine
title_full_unstemmed Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine
title_short Absence of p53 Mutations in Rat Colon Tumors Induced by 2‐Amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐Amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine
title_sort absence of p53 mutations in rat colon tumors induced by 2‐amino‐6‐methyldipyrido[1,2‐a:3′, 2′‐d]imidazole, 2‐amino‐3‐methylimidazo[4,5‐f]quinoline, or 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919493/
https://www.ncbi.nlm.nih.gov/pubmed/8014110
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02388.x
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