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Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines

We previously established a rapid and facile polymerase chain reaction (PCR)‐stop assay for quantitation of specific gene damage in very small numbers of cells. The present study investigated whether the PCR‐stop assay was able to detect variation in the extent of DNA damage in transcribed active ge...

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Detalles Bibliográficos
Autores principales: Oshita, Fumihiro, Saijo, Nagahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919540/
https://www.ncbi.nlm.nih.gov/pubmed/8071107
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02412.x
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author Oshita, Fumihiro
Saijo, Nagahiro
author_facet Oshita, Fumihiro
Saijo, Nagahiro
author_sort Oshita, Fumihiro
collection PubMed
description We previously established a rapid and facile polymerase chain reaction (PCR)‐stop assay for quantitation of specific gene damage in very small numbers of cells. The present study investigated whether the PCR‐stop assay was able to detect variation in the extent of DNA damage in transcribed active genes between cisplatin‐ or VP‐16‐resistant and sensitive cells. The assay demonstrated that about twice as much genetic damage occurs in PC‐9 cells than in cisplatin‐resistant PC‐9/CDDP cells following cisplatin exposure and about 4.6 times more damage occurs in H69 than in VP‐16‐resistant H69/VP cells following VP‐16 exposure. These results show that DNA damage, as detected by PCR‐stop assay, correlates with cytotoxicity. In conclusion, the PCR‐stop assay could be useful in detecting variation in DNA damage in specific genes.
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spelling pubmed-59195402018-05-11 Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines Oshita, Fumihiro Saijo, Nagahiro Jpn J Cancer Res Rapid Communication We previously established a rapid and facile polymerase chain reaction (PCR)‐stop assay for quantitation of specific gene damage in very small numbers of cells. The present study investigated whether the PCR‐stop assay was able to detect variation in the extent of DNA damage in transcribed active genes between cisplatin‐ or VP‐16‐resistant and sensitive cells. The assay demonstrated that about twice as much genetic damage occurs in PC‐9 cells than in cisplatin‐resistant PC‐9/CDDP cells following cisplatin exposure and about 4.6 times more damage occurs in H69 than in VP‐16‐resistant H69/VP cells following VP‐16 exposure. These results show that DNA damage, as detected by PCR‐stop assay, correlates with cytotoxicity. In conclusion, the PCR‐stop assay could be useful in detecting variation in DNA damage in specific genes. Blackwell Publishing Ltd 1994-07 /pmc/articles/PMC5919540/ /pubmed/8071107 http://dx.doi.org/10.1111/j.1349-7006.1994.tb02412.x Text en
spellingShingle Rapid Communication
Oshita, Fumihiro
Saijo, Nagahiro
Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines
title Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines
title_full Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines
title_fullStr Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines
title_full_unstemmed Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines
title_short Rapid Polymerase Chain Reaction Assay to Detect Variation in the Extent of Gene‐specific Damage between Cisplatin‐ or VP‐16‐resistant and Sensitive Lung Cancer Cell Lines
title_sort rapid polymerase chain reaction assay to detect variation in the extent of gene‐specific damage between cisplatin‐ or vp‐16‐resistant and sensitive lung cancer cell lines
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919540/
https://www.ncbi.nlm.nih.gov/pubmed/8071107
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02412.x
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