ELISA method to detect α-synuclein oligomers in cell and animal models

Soluble aggregates of α-synuclein, so-called oligomers, are hypothesized to act as neurotoxic species in Parkinson’s disease, Lewy body dementia and multiple systems atrophy, but specific tools to detect these aggregated species are only slowly appearing. We have developed an α-synuclein oligomer EL...

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Detalles Bibliográficos
Autores principales: Lassen, Louise Berkhoudt, Gregersen, Emil, Isager, Anne Kathrine, Betzer, Cristine, Kofoed, Rikke Hahn, Jensen, Poul Henning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919555/
https://www.ncbi.nlm.nih.gov/pubmed/29698510
http://dx.doi.org/10.1371/journal.pone.0196056
Descripción
Sumario:Soluble aggregates of α-synuclein, so-called oligomers, are hypothesized to act as neurotoxic species in Parkinson’s disease, Lewy body dementia and multiple systems atrophy, but specific tools to detect these aggregated species are only slowly appearing. We have developed an α-synuclein oligomer ELISA that allows us to detect and compare α-synuclein oligomer levels in different in vivo and in vitro experiments. The ELISA is based on commercially available antibodies and the epitope of the capture antibody MJF14-6-4-2 is folding- and aggregate-dependent and not present on monomers.

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