ELISA method to detect α-synuclein oligomers in cell and animal models

Soluble aggregates of α-synuclein, so-called oligomers, are hypothesized to act as neurotoxic species in Parkinson’s disease, Lewy body dementia and multiple systems atrophy, but specific tools to detect these aggregated species are only slowly appearing. We have developed an α-synuclein oligomer EL...

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Autores principales: Lassen, Louise Berkhoudt, Gregersen, Emil, Isager, Anne Kathrine, Betzer, Cristine, Kofoed, Rikke Hahn, Jensen, Poul Henning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919555/
https://www.ncbi.nlm.nih.gov/pubmed/29698510
http://dx.doi.org/10.1371/journal.pone.0196056
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author Lassen, Louise Berkhoudt
Gregersen, Emil
Isager, Anne Kathrine
Betzer, Cristine
Kofoed, Rikke Hahn
Jensen, Poul Henning
author_facet Lassen, Louise Berkhoudt
Gregersen, Emil
Isager, Anne Kathrine
Betzer, Cristine
Kofoed, Rikke Hahn
Jensen, Poul Henning
author_sort Lassen, Louise Berkhoudt
collection PubMed
description Soluble aggregates of α-synuclein, so-called oligomers, are hypothesized to act as neurotoxic species in Parkinson’s disease, Lewy body dementia and multiple systems atrophy, but specific tools to detect these aggregated species are only slowly appearing. We have developed an α-synuclein oligomer ELISA that allows us to detect and compare α-synuclein oligomer levels in different in vivo and in vitro experiments. The ELISA is based on commercially available antibodies and the epitope of the capture antibody MJF14-6-4-2 is folding- and aggregate-dependent and not present on monomers.
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spelling pubmed-59195552018-05-11 ELISA method to detect α-synuclein oligomers in cell and animal models Lassen, Louise Berkhoudt Gregersen, Emil Isager, Anne Kathrine Betzer, Cristine Kofoed, Rikke Hahn Jensen, Poul Henning PLoS One Research Article Soluble aggregates of α-synuclein, so-called oligomers, are hypothesized to act as neurotoxic species in Parkinson’s disease, Lewy body dementia and multiple systems atrophy, but specific tools to detect these aggregated species are only slowly appearing. We have developed an α-synuclein oligomer ELISA that allows us to detect and compare α-synuclein oligomer levels in different in vivo and in vitro experiments. The ELISA is based on commercially available antibodies and the epitope of the capture antibody MJF14-6-4-2 is folding- and aggregate-dependent and not present on monomers. Public Library of Science 2018-04-26 /pmc/articles/PMC5919555/ /pubmed/29698510 http://dx.doi.org/10.1371/journal.pone.0196056 Text en © 2018 Lassen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lassen, Louise Berkhoudt
Gregersen, Emil
Isager, Anne Kathrine
Betzer, Cristine
Kofoed, Rikke Hahn
Jensen, Poul Henning
ELISA method to detect α-synuclein oligomers in cell and animal models
title ELISA method to detect α-synuclein oligomers in cell and animal models
title_full ELISA method to detect α-synuclein oligomers in cell and animal models
title_fullStr ELISA method to detect α-synuclein oligomers in cell and animal models
title_full_unstemmed ELISA method to detect α-synuclein oligomers in cell and animal models
title_short ELISA method to detect α-synuclein oligomers in cell and animal models
title_sort elisa method to detect α-synuclein oligomers in cell and animal models
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919555/
https://www.ncbi.nlm.nih.gov/pubmed/29698510
http://dx.doi.org/10.1371/journal.pone.0196056
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