Semi‐quantitative Analysis of DNA Topoisomerase‐I mRNA Level Using Reverse Transcription‐Polymerase Chain Reaction in Cancer Cell Lines: Its Relation to Cytotoxicity against Camptothecin Derivative

Expression of DNA topoisomerase (Topo)‐I mRNA in various cancer cell lines was detected using the reverse transcription‐polymerase chain reaction (RT‐PCR) method. The cytoplasmic polyadenylated RNA isolated from cancer cell lines was reverse‐transcribed and the complementary DNA was amplified by PCR...

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Detalles Bibliográficos
Autores principales: Niwa, Kenji, Misao, Ryou, Hanabayashi, Takabiro, Morishita, Shigeo, Murase, Toshiko, Itoh, Miho, Itoh, Naoki, Mori, Hidehiro, Tamaya, Teruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1994
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919567/
https://www.ncbi.nlm.nih.gov/pubmed/7523352
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02960.x

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