Synthetic Oligonucleotides with Certain Palindromes Stimulate Interferon Production of Human Peripheral Blood Lymphocytes in vitro

We studied the ability of synthetic single‐stranded 30‐mer oligodeoxyribonucleotides oligoDNAs) with three different kinds of hexamer palindromic sequence to induce interferon (IFN) production of human peripheral blood lymphocytes (PBL). When PBL was cultured with oligoDNA having a palindrome of AAC...

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Detalles Bibliográficos
Autores principales: Yamamoto, Toshiko, Yamamoto, Saburo, Kataoka, Tetsuro, Komuro, Katsutoshi, Kohase, Masayoshi, Tokunaga, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1994
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919569/
https://www.ncbi.nlm.nih.gov/pubmed/7523351
http://dx.doi.org/10.1111/j.1349-7006.1994.tb02947.x
Descripción
Sumario:We studied the ability of synthetic single‐stranded 30‐mer oligodeoxyribonucleotides oligoDNAs) with three different kinds of hexamer palindromic sequence to induce interferon (IFN) production of human peripheral blood lymphocytes (PBL). When PBL was cultured with oligoDNA having a palindrome of AACGTT or GACGTC, IFN activity was detected by bioassay in the culture fluid after 8 h, and the amount of IFN reached the maximum after 18 h. IFN‐α was predominantly produced, and small amounts of IFN‐β and IFN‐γ were also found. OligoDNA with the palindrome ACCGGT had no effect.

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