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TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines
Gangliosides are glycosphingolipids concentrated in glycolipid-enriched membrane microdomains. Mainly restricted to the nervous system in healthy adult, complex gangliosides such as G(D3) and G(D2) have been shown to be involved in aggressiveness and metastasis of neuro-ectoderm derived tumors such...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919650/ https://www.ncbi.nlm.nih.gov/pubmed/29698439 http://dx.doi.org/10.1371/journal.pone.0196369 |
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author | Dewald, Justine H. Cavdarli, Sumeyye Steenackers, Agata Delannoy, Clément P. Mortuaire, Marlène Spriet, Corentin Noël, Maxence Groux-Degroote, Sophie Delannoy, Philippe |
author_facet | Dewald, Justine H. Cavdarli, Sumeyye Steenackers, Agata Delannoy, Clément P. Mortuaire, Marlène Spriet, Corentin Noël, Maxence Groux-Degroote, Sophie Delannoy, Philippe |
author_sort | Dewald, Justine H. |
collection | PubMed |
description | Gangliosides are glycosphingolipids concentrated in glycolipid-enriched membrane microdomains. Mainly restricted to the nervous system in healthy adult, complex gangliosides such as G(D3) and G(D2) have been shown to be involved in aggressiveness and metastasis of neuro-ectoderm derived tumors such as melanoma and neuroblastoma. GD3 synthase (GD3S), the key enzyme that controls the biosynthesis of complex gangliosides, was shown to be over-expressed in Estrogen Receptor (ER)-negative breast cancer tumors, and associated with a decreased overall survival of patients. We previously demonstrated that GD3S expression in ER-negative breast cancer cells induced a proliferative phenotype and an increased tumor growth. In addition, our results clearly indicate that Tumor Necrosis Factor (TNF) induced GD3S over-expression in breast cancer cells via NFκB pathway. In this study, we analyzed the effect of TNF on ganglioside biosynthesis and expression in breast cancer cells from different molecular subtypes. We showed that TNF up-regulated the expression of GD3S in MCF-7 and Hs578T cells, whereas no change was observed for MDA-MB-231. We also showed that TNF induced an increased expression of complex gangliosides at the cell surface of a small proportion of MCF-7 cells. These results demonstrate that TNF differentially regulates gangliosides expression in breast cancer cell lines and establish a possible link between inflammation at the tumor site environment, expression of complex gangliosides and tumor development. |
format | Online Article Text |
id | pubmed-5919650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59196502018-05-11 TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines Dewald, Justine H. Cavdarli, Sumeyye Steenackers, Agata Delannoy, Clément P. Mortuaire, Marlène Spriet, Corentin Noël, Maxence Groux-Degroote, Sophie Delannoy, Philippe PLoS One Research Article Gangliosides are glycosphingolipids concentrated in glycolipid-enriched membrane microdomains. Mainly restricted to the nervous system in healthy adult, complex gangliosides such as G(D3) and G(D2) have been shown to be involved in aggressiveness and metastasis of neuro-ectoderm derived tumors such as melanoma and neuroblastoma. GD3 synthase (GD3S), the key enzyme that controls the biosynthesis of complex gangliosides, was shown to be over-expressed in Estrogen Receptor (ER)-negative breast cancer tumors, and associated with a decreased overall survival of patients. We previously demonstrated that GD3S expression in ER-negative breast cancer cells induced a proliferative phenotype and an increased tumor growth. In addition, our results clearly indicate that Tumor Necrosis Factor (TNF) induced GD3S over-expression in breast cancer cells via NFκB pathway. In this study, we analyzed the effect of TNF on ganglioside biosynthesis and expression in breast cancer cells from different molecular subtypes. We showed that TNF up-regulated the expression of GD3S in MCF-7 and Hs578T cells, whereas no change was observed for MDA-MB-231. We also showed that TNF induced an increased expression of complex gangliosides at the cell surface of a small proportion of MCF-7 cells. These results demonstrate that TNF differentially regulates gangliosides expression in breast cancer cell lines and establish a possible link between inflammation at the tumor site environment, expression of complex gangliosides and tumor development. Public Library of Science 2018-04-26 /pmc/articles/PMC5919650/ /pubmed/29698439 http://dx.doi.org/10.1371/journal.pone.0196369 Text en © 2018 Dewald et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dewald, Justine H. Cavdarli, Sumeyye Steenackers, Agata Delannoy, Clément P. Mortuaire, Marlène Spriet, Corentin Noël, Maxence Groux-Degroote, Sophie Delannoy, Philippe TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines |
title | TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines |
title_full | TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines |
title_fullStr | TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines |
title_full_unstemmed | TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines |
title_short | TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines |
title_sort | tnf differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919650/ https://www.ncbi.nlm.nih.gov/pubmed/29698439 http://dx.doi.org/10.1371/journal.pone.0196369 |
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