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Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression
The present study investigated the possible tumor-suppressing function of microRNA (miR)-612 and the underlying molecular mechanism of its action in bladder cancer in vitro and in vivo. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was carried out to quantify the expression...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919718/ https://www.ncbi.nlm.nih.gov/pubmed/29620192 http://dx.doi.org/10.3892/ijo.2018.4342 |
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author | Liu, Mengnan Chen, Yifan Huang, Bisheng Mao, Shiyu Cai, Keke Wang, Longsheng Yao, Xudong |
author_facet | Liu, Mengnan Chen, Yifan Huang, Bisheng Mao, Shiyu Cai, Keke Wang, Longsheng Yao, Xudong |
author_sort | Liu, Mengnan |
collection | PubMed |
description | The present study investigated the possible tumor-suppressing function of microRNA (miR)-612 and the underlying molecular mechanism of its action in bladder cancer in vitro and in vivo. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was carried out to quantify the expression levels of miR-612 in bladder cancer tissues and cell lines. The data demonstrated that the level of miR-612 expression was significantly reduced in bladder cancer tissues and cell lines, as compared with that in non-cancerous tissues and cells. Reduced miR-612 expression was associated with advanced tumor, lymph node and metastasis stages, and with distant metastasis of bladder cancer. A functional study revealed that transfection of cells with an miR-612 mimic suppressed bladder cancer cell growth, colony formation, migration, invasion and epithelial-mesenchymal transition. Bioinformatics analysis identified that miR-612 targeted the expression of malic enzyme 1 (ME1), and this was confirmed by western blot and luciferase reporter assay results. Furthermore, the ME1 expression levels were inversely associated with miR-612 expression in bladder cancer tissue specimens. In addition, knockdown of ME1 expression using ME1 siRNA mimicked the effect of ectopic miR-612 overexpression in bladder cancer cells in terms of tumor cell growth, migration and invasion. By contrast, ME1 overexpression weakened the inhibitory effect of the miR-612 mimic in bladder cancer cells. In conclusion, the present study demonstrated that miR-612 may function as a tumor suppressor in bladder cancer by targeting ME1 expression. |
format | Online Article Text |
id | pubmed-5919718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-59197182018-05-03 Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression Liu, Mengnan Chen, Yifan Huang, Bisheng Mao, Shiyu Cai, Keke Wang, Longsheng Yao, Xudong Int J Oncol Articles The present study investigated the possible tumor-suppressing function of microRNA (miR)-612 and the underlying molecular mechanism of its action in bladder cancer in vitro and in vivo. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was carried out to quantify the expression levels of miR-612 in bladder cancer tissues and cell lines. The data demonstrated that the level of miR-612 expression was significantly reduced in bladder cancer tissues and cell lines, as compared with that in non-cancerous tissues and cells. Reduced miR-612 expression was associated with advanced tumor, lymph node and metastasis stages, and with distant metastasis of bladder cancer. A functional study revealed that transfection of cells with an miR-612 mimic suppressed bladder cancer cell growth, colony formation, migration, invasion and epithelial-mesenchymal transition. Bioinformatics analysis identified that miR-612 targeted the expression of malic enzyme 1 (ME1), and this was confirmed by western blot and luciferase reporter assay results. Furthermore, the ME1 expression levels were inversely associated with miR-612 expression in bladder cancer tissue specimens. In addition, knockdown of ME1 expression using ME1 siRNA mimicked the effect of ectopic miR-612 overexpression in bladder cancer cells in terms of tumor cell growth, migration and invasion. By contrast, ME1 overexpression weakened the inhibitory effect of the miR-612 mimic in bladder cancer cells. In conclusion, the present study demonstrated that miR-612 may function as a tumor suppressor in bladder cancer by targeting ME1 expression. D.A. Spandidos 2018-03-29 /pmc/articles/PMC5919718/ /pubmed/29620192 http://dx.doi.org/10.3892/ijo.2018.4342 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Mengnan Chen, Yifan Huang, Bisheng Mao, Shiyu Cai, Keke Wang, Longsheng Yao, Xudong Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression |
title | Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression |
title_full | Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression |
title_fullStr | Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression |
title_full_unstemmed | Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression |
title_short | Tumor-suppressing effects of microRNA-612 in bladder cancer cells by targeting malic enzyme 1 expression |
title_sort | tumor-suppressing effects of microrna-612 in bladder cancer cells by targeting malic enzyme 1 expression |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919718/ https://www.ncbi.nlm.nih.gov/pubmed/29620192 http://dx.doi.org/10.3892/ijo.2018.4342 |
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