The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification

A 60-year-old man developed pneumonia after undergoing autologous peripheral blood stem cell transplantation for diffuse large-B cell lymphoma. A urinary antigen test and sputum culture were both negative for Legionella pneumophila; however, a sputum sample that was examined by loop-mediated isother...

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Autores principales: Moriguchi, Shuhei, Abe, Masahiro, Kimura, Muneyoshi, Yoshino, Chiemi, Baba, Masaru, Okada, Chikako, Izutsu, Koji, Taniguchi, Shuichi, Araoka, Hideki, Yoneyama, Akiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Internal Medicine 2017
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919869/
https://www.ncbi.nlm.nih.gov/pubmed/29269681
http://dx.doi.org/10.2169/internalmedicine.9810-17
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author Moriguchi, Shuhei
Abe, Masahiro
Kimura, Muneyoshi
Yoshino, Chiemi
Baba, Masaru
Okada, Chikako
Izutsu, Koji
Taniguchi, Shuichi
Araoka, Hideki
Yoneyama, Akiko
author_facet Moriguchi, Shuhei
Abe, Masahiro
Kimura, Muneyoshi
Yoshino, Chiemi
Baba, Masaru
Okada, Chikako
Izutsu, Koji
Taniguchi, Shuichi
Araoka, Hideki
Yoneyama, Akiko
author_sort Moriguchi, Shuhei
collection PubMed
description A 60-year-old man developed pneumonia after undergoing autologous peripheral blood stem cell transplantation for diffuse large-B cell lymphoma. A urinary antigen test and sputum culture were both negative for Legionella pneumophila; however, a sputum sample that was examined by loop-mediated isothermal amplification (LAMP) was positive for Legionella spp. On admission, the results of blood culturing using a BACTEC system were negative for 7 days. However, L. pneumophila serogroup 5 was detected in a blood subculture using WYOα medium. The patient was successfully treated with a fluoroquinolone-based regimen. LAMP is useful for the diagnosis of Legionella spp.
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spelling pubmed-59198692018-04-27 The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification Moriguchi, Shuhei Abe, Masahiro Kimura, Muneyoshi Yoshino, Chiemi Baba, Masaru Okada, Chikako Izutsu, Koji Taniguchi, Shuichi Araoka, Hideki Yoneyama, Akiko Intern Med Case Report A 60-year-old man developed pneumonia after undergoing autologous peripheral blood stem cell transplantation for diffuse large-B cell lymphoma. A urinary antigen test and sputum culture were both negative for Legionella pneumophila; however, a sputum sample that was examined by loop-mediated isothermal amplification (LAMP) was positive for Legionella spp. On admission, the results of blood culturing using a BACTEC system were negative for 7 days. However, L. pneumophila serogroup 5 was detected in a blood subculture using WYOα medium. The patient was successfully treated with a fluoroquinolone-based regimen. LAMP is useful for the diagnosis of Legionella spp. The Japanese Society of Internal Medicine 2017-12-21 2018-04-01 /pmc/articles/PMC5919869/ /pubmed/29269681 http://dx.doi.org/10.2169/internalmedicine.9810-17 Text en Copyright © 2018 by The Japanese Society of Internal Medicine https://creativecommons.org/licenses/by-nc-nd/4.0/ The Internal Medicine is an Open Access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. To view the details of this license, please visit (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Case Report
Moriguchi, Shuhei
Abe, Masahiro
Kimura, Muneyoshi
Yoshino, Chiemi
Baba, Masaru
Okada, Chikako
Izutsu, Koji
Taniguchi, Shuichi
Araoka, Hideki
Yoneyama, Akiko
The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification
title The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification
title_full The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification
title_fullStr The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification
title_full_unstemmed The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification
title_short The Diagnosis of Legionella pneumophila Serogroup 5 Bacteremic Pneumonia during Severe Neutropenia Using Loop-mediated Isothermal Amplification
title_sort diagnosis of legionella pneumophila serogroup 5 bacteremic pneumonia during severe neutropenia using loop-mediated isothermal amplification
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5919869/
https://www.ncbi.nlm.nih.gov/pubmed/29269681
http://dx.doi.org/10.2169/internalmedicine.9810-17
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