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Ancestry inference of 96 population samples using microhaplotypes
Microhaplotypes have become a new type of forensic marker with a great ability to identify and deconvolute mixtures because massively parallel sequencing (MPS) allows the alleles (haplotypes) of the multi-SNP loci to be determined directly for an individual. As originally defined, a microhaplotype l...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920014/ https://www.ncbi.nlm.nih.gov/pubmed/29248957 http://dx.doi.org/10.1007/s00414-017-1748-6 |
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author | Bulbul, Ozlem Pakstis, Andrew J. Soundararajan, Usha Gurkan, Cemal Brissenden, Jane E. Roscoe, Janet M. Evsanaa, Baigalmaa Togtokh, Ariunaa Paschou, Peristera Grigorenko, Elena L. Gurwitz, David Wootton, Sharon Lagace, Robert Chang, Joseph Speed, William C. Kidd, Kenneth K. |
author_facet | Bulbul, Ozlem Pakstis, Andrew J. Soundararajan, Usha Gurkan, Cemal Brissenden, Jane E. Roscoe, Janet M. Evsanaa, Baigalmaa Togtokh, Ariunaa Paschou, Peristera Grigorenko, Elena L. Gurwitz, David Wootton, Sharon Lagace, Robert Chang, Joseph Speed, William C. Kidd, Kenneth K. |
author_sort | Bulbul, Ozlem |
collection | PubMed |
description | Microhaplotypes have become a new type of forensic marker with a great ability to identify and deconvolute mixtures because massively parallel sequencing (MPS) allows the alleles (haplotypes) of the multi-SNP loci to be determined directly for an individual. As originally defined, a microhaplotype locus is a short segment of DNA with two or more SNPs defining three or more haplotypes. The length is short enough, less than about 300 bp, that the read length of current MPS technology can produce a phase-known sequence of each chromosome of an individual. As part of the discovery phase of our studies, data on 130 microhaplotype loci with estimates of haplotype frequency data on 83 populations have been published. To provide a better picture of global allele frequency variation, we have now tested 13 more populations for 65 of the microhaplotype loci from among those with higher levels of inter-population gene frequency variation, including 8 loci not previously published. These loci provide clear distinctions among 6 biogeographic regions and provide some information distinguishing up to 10 clusters of populations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00414-017-1748-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5920014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-59200142018-05-01 Ancestry inference of 96 population samples using microhaplotypes Bulbul, Ozlem Pakstis, Andrew J. Soundararajan, Usha Gurkan, Cemal Brissenden, Jane E. Roscoe, Janet M. Evsanaa, Baigalmaa Togtokh, Ariunaa Paschou, Peristera Grigorenko, Elena L. Gurwitz, David Wootton, Sharon Lagace, Robert Chang, Joseph Speed, William C. Kidd, Kenneth K. Int J Legal Med Original Article Microhaplotypes have become a new type of forensic marker with a great ability to identify and deconvolute mixtures because massively parallel sequencing (MPS) allows the alleles (haplotypes) of the multi-SNP loci to be determined directly for an individual. As originally defined, a microhaplotype locus is a short segment of DNA with two or more SNPs defining three or more haplotypes. The length is short enough, less than about 300 bp, that the read length of current MPS technology can produce a phase-known sequence of each chromosome of an individual. As part of the discovery phase of our studies, data on 130 microhaplotype loci with estimates of haplotype frequency data on 83 populations have been published. To provide a better picture of global allele frequency variation, we have now tested 13 more populations for 65 of the microhaplotype loci from among those with higher levels of inter-population gene frequency variation, including 8 loci not previously published. These loci provide clear distinctions among 6 biogeographic regions and provide some information distinguishing up to 10 clusters of populations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00414-017-1748-6) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-12-16 2018 /pmc/articles/PMC5920014/ /pubmed/29248957 http://dx.doi.org/10.1007/s00414-017-1748-6 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Bulbul, Ozlem Pakstis, Andrew J. Soundararajan, Usha Gurkan, Cemal Brissenden, Jane E. Roscoe, Janet M. Evsanaa, Baigalmaa Togtokh, Ariunaa Paschou, Peristera Grigorenko, Elena L. Gurwitz, David Wootton, Sharon Lagace, Robert Chang, Joseph Speed, William C. Kidd, Kenneth K. Ancestry inference of 96 population samples using microhaplotypes |
title | Ancestry inference of 96 population samples using microhaplotypes |
title_full | Ancestry inference of 96 population samples using microhaplotypes |
title_fullStr | Ancestry inference of 96 population samples using microhaplotypes |
title_full_unstemmed | Ancestry inference of 96 population samples using microhaplotypes |
title_short | Ancestry inference of 96 population samples using microhaplotypes |
title_sort | ancestry inference of 96 population samples using microhaplotypes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920014/ https://www.ncbi.nlm.nih.gov/pubmed/29248957 http://dx.doi.org/10.1007/s00414-017-1748-6 |
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