Cargando…

ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences

Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antige...

Descripción completa

Detalles Bibliográficos
Autores principales: Shanthamurthy, Chethan D., Jain, Prashant, Yehuda, Sharon, Monteiro, João T., Leviatan Ben-Arye, Shani, Subramani, Balamurugan, Lepenies, Bernd, Padler-Karavani, Vered, Kikkeri, Raghavendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920051/
https://www.ncbi.nlm.nih.gov/pubmed/29700341
http://dx.doi.org/10.1038/s41598-018-24333-y
_version_ 1783317754254721024
author Shanthamurthy, Chethan D.
Jain, Prashant
Yehuda, Sharon
Monteiro, João T.
Leviatan Ben-Arye, Shani
Subramani, Balamurugan
Lepenies, Bernd
Padler-Karavani, Vered
Kikkeri, Raghavendra
author_facet Shanthamurthy, Chethan D.
Jain, Prashant
Yehuda, Sharon
Monteiro, João T.
Leviatan Ben-Arye, Shani
Subramani, Balamurugan
Lepenies, Bernd
Padler-Karavani, Vered
Kikkeri, Raghavendra
author_sort Shanthamurthy, Chethan D.
collection PubMed
description Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca(2+)-dependent fashion.
format Online
Article
Text
id pubmed-5920051
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-59200512018-05-01 ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences Shanthamurthy, Chethan D. Jain, Prashant Yehuda, Sharon Monteiro, João T. Leviatan Ben-Arye, Shani Subramani, Balamurugan Lepenies, Bernd Padler-Karavani, Vered Kikkeri, Raghavendra Sci Rep Article Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca(2+)-dependent fashion. Nature Publishing Group UK 2018-04-26 /pmc/articles/PMC5920051/ /pubmed/29700341 http://dx.doi.org/10.1038/s41598-018-24333-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shanthamurthy, Chethan D.
Jain, Prashant
Yehuda, Sharon
Monteiro, João T.
Leviatan Ben-Arye, Shani
Subramani, Balamurugan
Lepenies, Bernd
Padler-Karavani, Vered
Kikkeri, Raghavendra
ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences
title ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences
title_full ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences
title_fullStr ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences
title_full_unstemmed ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences
title_short ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences
title_sort abo antigens active tri- and disaccharides microarray to evaluate c-type lectin receptor binding preferences
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920051/
https://www.ncbi.nlm.nih.gov/pubmed/29700341
http://dx.doi.org/10.1038/s41598-018-24333-y
work_keys_str_mv AT shanthamurthychethand aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT jainprashant aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT yehudasharon aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT monteirojoaot aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT leviatanbenaryeshani aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT subramanibalamurugan aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT lepeniesbernd aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT padlerkaravanivered aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences
AT kikkeriraghavendra aboantigensactivetrianddisaccharidesmicroarraytoevaluatectypelectinreceptorbindingpreferences