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3D Reconstruction of Lipid Droplets in the Seed of Brassica napus

Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details...

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Autores principales: Yin, Yongtai, Guo, Liangxing, Chen, Kang, Guo, Zhenyi, Chao, Hongbo, Wang, Baoshan, Li, Maoteng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920073/
https://www.ncbi.nlm.nih.gov/pubmed/29700334
http://dx.doi.org/10.1038/s41598-018-24812-2
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author Yin, Yongtai
Guo, Liangxing
Chen, Kang
Guo, Zhenyi
Chao, Hongbo
Wang, Baoshan
Li, Maoteng
author_facet Yin, Yongtai
Guo, Liangxing
Chen, Kang
Guo, Zhenyi
Chao, Hongbo
Wang, Baoshan
Li, Maoteng
author_sort Yin, Yongtai
collection PubMed
description Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details might have been lost in previous studies. In the present work, the configuration of LDs in seeds was obtained by confocal imaging combined with 3D reconstruction technology in Brassica napus. The size and shape of LDs, LD numbers, cell interval spaces and cell size were observed and compared at 3D levels in the seeds of different materials with high and low oil content. It was also revealed that different cells located in the same tissue exhibited various oil contents according to the construction at the 3D level, which was not previously reported in B. napus. The present work provides a new way to understand the differential in cell populations and enhance the seed oil content at the single cell level within seeds.
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spelling pubmed-59200732018-05-01 3D Reconstruction of Lipid Droplets in the Seed of Brassica napus Yin, Yongtai Guo, Liangxing Chen, Kang Guo, Zhenyi Chao, Hongbo Wang, Baoshan Li, Maoteng Sci Rep Article Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details might have been lost in previous studies. In the present work, the configuration of LDs in seeds was obtained by confocal imaging combined with 3D reconstruction technology in Brassica napus. The size and shape of LDs, LD numbers, cell interval spaces and cell size were observed and compared at 3D levels in the seeds of different materials with high and low oil content. It was also revealed that different cells located in the same tissue exhibited various oil contents according to the construction at the 3D level, which was not previously reported in B. napus. The present work provides a new way to understand the differential in cell populations and enhance the seed oil content at the single cell level within seeds. Nature Publishing Group UK 2018-04-26 /pmc/articles/PMC5920073/ /pubmed/29700334 http://dx.doi.org/10.1038/s41598-018-24812-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yin, Yongtai
Guo, Liangxing
Chen, Kang
Guo, Zhenyi
Chao, Hongbo
Wang, Baoshan
Li, Maoteng
3D Reconstruction of Lipid Droplets in the Seed of Brassica napus
title 3D Reconstruction of Lipid Droplets in the Seed of Brassica napus
title_full 3D Reconstruction of Lipid Droplets in the Seed of Brassica napus
title_fullStr 3D Reconstruction of Lipid Droplets in the Seed of Brassica napus
title_full_unstemmed 3D Reconstruction of Lipid Droplets in the Seed of Brassica napus
title_short 3D Reconstruction of Lipid Droplets in the Seed of Brassica napus
title_sort 3d reconstruction of lipid droplets in the seed of brassica napus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920073/
https://www.ncbi.nlm.nih.gov/pubmed/29700334
http://dx.doi.org/10.1038/s41598-018-24812-2
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