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Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells

Imaging the actin cytoskeleton in cells uses a wide range of approaches. Typically, a fluorescent derivative of the small cyclic peptide phalloidin is used to image F-actin in fixed cells. Lifeact and F-tractin are popular for imaging the cytoskeleton in live cells. Here we characterised novel affin...

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Detalles Bibliográficos
Autores principales:	Lopata, Anna, Hughes, Ruth, Tiede, Christian, Heissler, Sarah M., Sellers, James R., Knight, Peter J., Tomlinson, Darren, Peckham, Michelle
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920084/ https://www.ncbi.nlm.nih.gov/pubmed/29700342 http://dx.doi.org/10.1038/s41598-018-24953-4

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